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- We also suceeded in culturing 615 ES cell colonies by BRL conditioned medium. 615小鼠的ES细胞集落。 采用BRL条件培养基对分离的615小鼠ES细胞
- Studies on the Conditioned Media in the Direct Culture of Microspores in Barley. 大麦小孢子直接培养中条件因子的应用研究。
- Conditioned media from LX-2 cells treated by Cpd 861 for 24, 48, 72 h werecollected. 1 复方 861 对 LX-2 细胞增殖的影响结果表明,复方 861 对 LX-2 细胞增殖的影响与药物剂量相关。 在
- Objective To observe the human dermal papilla cells conditioned medium treat alopecia areata. 目的:人毛乳头细胞条件培养液治疗斑秃患者的临床观察。
- ES cells should be cultivated on MEF feeder layer or in BRL conditioned medium plus MEF feeder layer at the early stage after recovering. 在复苏后早期阶段ES细胞培养中 ,不宜单独应用BRL条件培养基 ,须用MEF饲养层或合并应用BRL条件培养基和MEF饲养层
- Each group was cultured separately with various conditioned media collected respectively as hypothalamus-pituitary(HP),hypothalamus-pituitary induced by diamide(dHP),and control. 每组又再分为 3小组 :HP组、dHP组、Control组(对照组 )。 分别培养于预先收集的下丘脑 垂体 (HP组 )、联胺诱导下丘脑 垂体 (dHP组 )和对照组 (Control组 )条件培养液。
- The effect of Leukemia-associated inhibitor (LAI) in leukemia cells conditioned media (LCM) and sera from patients with ANLL on normal T lymphocyte colony formation were studied. 通过收集16例急性非淋巴细胞白血病(ANLL)病人的血清和外周血白血病细胞条件培养液(LCM),对血清和LCM中的白血病相关抑制物(LAI)对正常人T淋巴细胞集落(CFU-TL)形成的影响进行研究。
- The invasiveness of trophoblast derived from first trimester pregnancy can be inhibited by decidual cell conditioned media (DCM),while the mechanism is not very clear. 先前研究表明,蜕膜细胞条件培养液(decidual cell conditioned media,DCM)能够阻止早孕滋养细胞的侵入,然而其具体机制尚未阐明。
- Bystander effects on unirradiated V79 cells were observed by irradiated conditioned medium (ICM) method and co? cultured with carbon? ion? 用经过碳离子辐照V79细胞的条件培养基(Irradiatedconditionedmedium,简称ICM)和与受照射细胞共同培养两种方法,研究了未受照射细胞的旁观者效应。
- Yang J, Zhang LS, Wu L, et al.Effects of conditioned medium of fibroblast on the growth and proliferation of Schwann cells [J].Chin J Clin Rehabil, 2004, 8(35): 7956-7. [2]杨俊;张黎声;武雷;等.;成纤维细胞条件培养基对雪旺细胞生长和增殖的影响[J]
- Methods NOV gene recombinant plasmid was respectively transfected into COS 7 cells and NSCs by liposome method. Conditioned media(CM) of COS 7 cells and COS 7 cells modified by NOV gene were collected. COS CM and NOV CM, NSCs were cultured in CM. 方法 NOV基因重组质粒转染COS 7细胞和NSCs ,收集COS 7细胞和NOV基因修饰COS 7细胞的条件培养液 (简称COS CM和NOV CM ) ,作用于培养的NSCs ,3H TdR掺入检测NSCs的增殖 ,免疫细胞化学和流式细胞仪检测NSCs的分化。
- The mechanism of the regulation of mouse hemopoiesis by the conditioned media of the peritoneal macrophage is discussed. Our results show that this supernatant of cultured peritoneal macrophages may contain EPO-like, BPA-like and CSF-GM-like factors. 本研究初步探讨了腹腔巨噬细胞培养上清液的造血调控机理,提示腹腔巨噬细胞培养上清液有促红细胞生成素(EPO)、爆式集落刺激活性(BPA)、粒-单核系集落刺激因子(CSF-GM)样活性,LPS可使巨噬细胞培养上清液中EPO、BPA和CSF-GM样活性增强。
- These results indicate that BFF has a significantly inhibitory effect on the in vitro fertilization of bovine oocytes, but the thecal cell conditioned medium can improve the fertilization quality of bovine oocytes. 这些结果表明:BFF对牛卵母细胞的体外受精具抑制作用,而内膜细胞的条件液则具促进效应。
- The colonies of HPP CFC were supported by IL 1,IL 6, WEHI 3 conditioned medium (WEHI 3 CM, which contained IL 3) and L929 conditioned medium (L929 CM,which contained M CSF); the colonies of CFU GM were supported by WEHI 3 CM. HPP-CFC集落由IL-1、IL-6、WEHI3条件培养液(WEHI3-CM,含有IL-3)及L929条件培养液(L929-CM,含有M-CSF)所支持,而CFU-GM由WEHI3-CM所支持。
- EPCs paracrine function was examined by BrdU staining of ECs which were stimulated with EPCs conditional medium. EPCs的旁分泌功能测定采用EPCs条件培养液刺激ECs,并用BrdU法测定ECs核DNA复制。
- Conditioned medium (CM) from cultured NOV/COS-7 cells (NOV-CM) was collected, it markedly promoted the incorporation of JH-TdR in cultured NSCs, the effect was concentration-dependent, this indicated that NOV-CM promoted proliferation of NSCs. 收集培养NOV/COS、7细胞的条件培养液(NO-CM),发现NOV-CM能明显促进 NSCSsH1dR的摄入,且其作用存在一定的量效关系,提示NOVCM能刺激NSCS的增殖;
- Converselly, Bep, W 7 and TMB B decreased the NGF content in the conditioned medium and decreased the stimulation effect of HC on the NGF synthesis in culture mouse astroglial cell. 同时还可不同程度地减弱HC对静止期小鼠星形细胞合成NGF的促进作用。
- In the present study, the effects of murine bone marrow endothelial cell conditioned medium (ECM) combined with flt3 ligand (FL) or/and thrombopoietin (TPO) on the proliferation of HPP CFC and CFU GM were investigated. 通过传代培养小鼠骨髓内皮细胞 ,收集无血清条件培养液 (ECM) ,并经超滤得到大于 10kD的浓缩液 ,分别观察ECM和大于 10kD的浓缩液复合flt3ligand (FL)及thrombopoietin (TPO)对体外培养HPP CFC、CFU GM的影响。
- English is not the usual medium of instruction in our school. 英语不是我校通常使用的教学语言。
- Methods:EC and SMC were isolated from rabbit aortas and cultured. The conditioned medium(CM) of EC and SMC were harvested. ECCM,ECCM conditioned in the presence of Phosphoramidon,SMCCM and ET-1 were added individually to the cultures. 方法 :EC和 SMC均来源于兔主动脉 ,在获得了 EC和SMC的条件培养基 (CM)后 ,分别用两者以及 ET- 1进行实验 ,细胞的增殖率通过 3H掺入法进行测定。