Methods:EC and SMC were isolated from rabbit aortas and cultured. The conditioned medium(CM) of EC and SMC were harvested. ECCM,ECCM conditioned in the presence of Phosphoramidon,SMCCM and ET-1 were added individually to the cultures.

 
  • 方法 :EC和 SMC均来源于兔主动脉 ,在获得了 EC和SMC的条件培养基 (CM)后 ,分别用两者以及 ET- 1进行实验 ,细胞的增殖率通过 3H掺入法进行测定。
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