In the present study, the effects of murine bone marrow endothelial cell conditioned medium (ECM) combined with flt3 ligand (FL) or/and thrombopoietin (TPO) on the proliferation of HPP CFC and CFU GM were investigated.

• 通过传代培养小鼠骨髓内皮细胞 ,收集无血清条件培养液 (ECM) ,并经超滤得到大于 10kD的浓缩液 ,分别观察ECM和大于 10kD的浓缩液复合flt3ligand (FL)及thrombopoietin (TPO)对体外培养HPP CFC、CFU GM的影响。