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- The recombinant plasmid pBV220-Arr was construted successfully and the target protein Arresten can express in E. 成功构建Arresten基因重组质粒pBV220-Arr;并可在E.
- The result showed the target protein was successful expressed as a SLH-Ap36 fusion protein. 结果表明,目标蛋白以融合蛋白的形式成功表达。
- After purification, the target protein further renaturation 2 - BMP via degeneration dissolving, load dialysis bag. 纯化后的的目的蛋白进一步复性,BMP-2经变性液溶解,装入透析袋内。
- The reconstructed vector NY-ESO-1-pET-28 could express target protein stably in the soluble fraction of bacterial extract. 构建的原核表达载体NY-ESO-1-pET28a(+)可稳定地可溶性表达NY-ESO-1蛋白;
- Some phages showing low, medium, high specificity to each target protein, lysozyme, insulin and RNase A, had been selected respectively for sequencing. 利用新建立的交替洗脱法,使用其它目标蛋白质对噬菌体展示肽库进行筛选,又获得了对胰岛素和核糖核酸酶A具有高亲和力的噬菌体。
- By expression vector pHHSM002, tpsl gene also expressed efficiently in E. coli BL21(DE3)PlysS ,the target protein amount to 17.8% in cell. 以pHHSM002为表达载体,tps1基因在大肠杆菌BL21中也得到了高效表达,表达的目的蛋白占菌体总蛋白的17.;8%25。
- Pharmacophore hypothesis(PH) is fundamental to de novo design of peptide ligand based on the structure of target protein. 在基于靶蛋白结构的多肽分子设计中,药效假说是从头设计方法的基本前提。
- Other uses of microarrays include the screening of large numbers of molecules for their capacity to bind a particular target protein. 其它微点阵的用途包括从大量分子中筛选出能结合特定靶蛋白的分子。
- SUMO protease can cut SUMO fusion protein expressed by fusion expression system without any amino acid residues left on target protein thus become a hot topic in this field. SUMO蛋白酶对SUMO融合表达系统表达的重组蛋白进行切割时没有多余氨基酸残留,因此成为蛋白切割工具的热点。
- Using different density urea to isolate the inclusion body, then purify the target protein through the Ni-NTA resin, analyzed with SDS-PAGE found the purity attained over 90%. 用尿素梯度分离包涵体,确定1 M尿素洗涤包涵体为分离MP-GFP的最优条件。经Ni-NTA树脂纯化目标蛋白,SDS-PAGE电泳分析其纯度达到90%25以上。
- SDS-PAGE results showed that there was a clear target protein band in Mut+ recombinant supernatant after 48 hours of culturing, while a faint band only in Muts recombinant after 72 hours. 结果表明,诱导培养48小时后,Mut~+重组菌株表达产物在SDS-PAGE胶上显现出清晰的目的蛋白带,而Mut~s重组菌株培养72小时才能显示微弱的目的带;
- Firstly, I subcloned the DNA fragment that encode the four consensus repeat of DAF into the prokaryotic expression vector pET32a(+) which could add a Trx tag on the N-terminal of the target protein. 首先通过PCR的方法,将编码大鼠DAF四个SCR的基因片段亚克隆到Trx融合原核表达载体pET32a(+)。
- Crystal structure analysis of drug target proteins complexed with small chemicals. 药物靶标蛋白与小分子化合物的复合物晶体结构研究。
- The eukaryotic expression plasmid pcD85B was constructed. The recombinantplsmid was transfected into COS-7 cells and the expressed target protein was tested byRT-PCR, ELISA and dot blotting. pcD3.;1(+)、pcD85B、转染 COS-7 细胞;用 RT-PCR、ELISA 和斑点印迹的方 法鉴定目的基因的表达;
- FBC showed very high affinity to all three essential target proteins in the membrane of K799/WT. 结果表明呋苄青霉素对绿脓杆菌K799/WT的致死性PBPs具有强大的亲和力。
- The expressed protein was purified by Glutath-one Sepharose 4B.A lot of target protein and a little of GST protein were obtained,the density of M2 was 5.6 mg/mL after defining the purification. 表达产物使用GST亲和吸附柱进行纯化;获得了大量的目的蛋白及微量的GST蛋白;使用分光光度仪确定纯化后M2蛋白的浓度约为5.;6 mg/mL。
- Microsomal fraction of grape fruit are isolated, separated on a SDS-PAGE, then eluted the target protein from the gel. lyophilizing and gathering, we got the protein of the electrophoretically purity. 制备葡萄果实微粒体,经过大量的SDS-PAGE电泳,将目的蛋白从胶上洗下来,低压冷冻干燥、富集,得到了电泳纯的目的蛋白。
- Inclusion bodies were solubilized in 8 mol/L urea,and purified directly by immobilized metal ion affinity chromatography (IMAC), purification product was target protein by SDS PAGE analysis. 用 8mol/L尿素溶解包涵体后 ,利用镍离子金属螯合亲和层析法纯化蛋白。
- The recombinant plsmids were transfected into COS-7 cells respectively and to test the expressed target proteins by RT-PCR, ELISA and dot blotting. pcDNA3.;1(+)、pcDNA/Ag85B、pcDNA/MPT64和pcDNA/AM转染COS-7细胞,用RT-PCR、ELISA和斑点印迹的方法鉴定目的基因的表达;