The expressed protein was purified by Glutath-one Sepharose 4B.A lot of target protein and a little of GST protein were obtained,the density of M2 was 5.6 mg/mL after defining the purification.

• 表达产物使用GST亲和吸附柱进行纯化；获得了大量的目的蛋白及微量的GST蛋白；使用分光光度仪确定纯化后M2蛋白的浓度约为5.；6 mg/mL。