Using different density urea to isolate the inclusion body, then purify the target protein through the Ni-NTA resin, analyzed with SDS-PAGE found the purity attained over 90%.

• 用尿素梯度分离包涵体，确定1 M尿素洗涤包涵体为分离MP-GFP的最优条件。经Ni-NTA树脂纯化目标蛋白，SDS-PAGE电泳分析其纯度达到90%25以上。