The recombinant plsmids were transfected into COS-7 cells respectively and to test the expressed target proteins by RT-PCR, ELISA and dot blotting.
英
美
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pcDNA3.;1(+)、pcDNA/Ag85B、pcDNA/MPT64和pcDNA/AM转染COS-7细胞,用RT-PCR、ELISA和斑点印迹的方法鉴定目的基因的表达;
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