Firstly, I subcloned the DNA fragment that encode the four consensus repeat of DAF into the prokaryotic expression vector pET32a(+) which could add a Trx tag on the N-terminal of the target protein.

• 首先通过PCR的方法，将编码大鼠DAF四个SCR的基因片段亚克隆到Trx融合原核表达载体pET32a(+)。