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The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 将该cDNA插入原核表达载体pET3d并在大肠杆菌BL21(DE3)中过量表达。
Conclusion: The prokaryotic expression vector with target gene was constructed successfully. 结论:成功构建了带有目的基因的原核表达载体。
Full-length or truncated cDNA was subcloned into prokaryotic expression vector pET30a and expression induced in E. coli BL21(DE3). 全长的或C末端截短的鲨烯合酶cDNA被克隆进原核表达载体pET30a并在大肠杆菌BL21(DE3)中诱导表达。
Then the BLY cDNA fragment was subcloned into prokaryotic expression vector pGEX-4T-1, forming the prokaryotic expression plasmid (pG-BLY). 克隆PCR产物，并构建了pGEX-4T-1-BLY原核表达载体。经BamHI和EcoRI酶切及质粒PCR鉴定，证实本实验构建的新型牛溶菌酶基因已克隆到原核表达载体pGEX-4T-1上，为进一步研究其诱导表达条件及生物学功能奠定了基础。
The choEW was inserted into prokaryotic expression vector pET-His, and the resulting recombinant plasmid pETW was used to transform E. colt BL21(DE3)plysS. 将choEW插入到原核表达载体pET-His中,构建出重组质粒pETW,并转化Escherichia coli BL21(DE3)plysS获得工程菌。
Inducible prokaryotic expression vector pET-Nus-STK11(with Nus fusion tag) was constructed with pET-44a(+) and the cDNA of STK11 gene cloned in our lab. 利用本室克隆的人STK11 cDNA和原核表达载体pET-44a(+)构建带有Nus融合标签的诱导型表达载体pET-Nus-STK11,在不同的大肠杆菌宿主中诱导表达。
Methods:(CTP)_4coding gene from pBSMR-(CTP)_4 was cloned into prokaryotic expression vector pET-28a(+) and the expression plasmid pET-28a(+)- (CTP)_4was obtained. 方法:将pBSMR-(CTP)_4中的(CTP)_4基因片断克隆入表达载体pET-28a(+),得到表达质粒pET-28a(+)-(CTP)_4。
The identified cDNA was cloned into the new type of prokaryotic expression vector pQE-80L, and a synthetized expression plasmid named pQE-80L/DHFR/ABP obtained. The E. 将此基因克隆到原核表达载体pQE-80L,获得融合表达质粒pQE-80L/DHFR/ABP,在1%25IPTG诱导下进行表达。
Mouse obese gene was amplified by PCR and subcloned into prokaryotic expression vector pET-28a(+) to construct the recombinant pET-28a-lep. Transforming pET-28a-lep into E. 本研究以克隆有小鼠肥胖基因的质粒pMET mouse leptin为模板，PCR扩增小鼠的ob基因，定向克隆至原核表达载体pET-28a(+)，构建重组表达质粒pET-28a-lep。
Subsequently, the P36 gene of Mhp strain ZCF23 was subcloned into the prokaryotic expression vector pGEX 6P-1 through EcoR I and Xho I double digestion, then the recombinant expression vector pGEX 6P-1-P36 was constructed and transformed into E. 序列确认后，用Ec口Rl和肠口I将P36基因从克隆载体中切出，并插入到GsT融和表达载体pGEx 6p一1相应位点，构建成重组表达载体pGEX 6p一1一P36，并转化宿主菌BLZI。阳性克隆命名为BLZI(6P一l一P36)。
Linoleate isomerase(LI ) gene was amplified by PCR from chromosome of Lactobacillus reuteri PYR8,and was inserted into pET30a to construct the prokaryotic expression vector pET30a-LI. 以Lactobacillus reuteri PYR8菌株基因组为模板,利用PCR方法扩增出亚油酸异构酶(linoleate isomerase,LI)基因,亚克隆到pET30a中构建原核表达载体pET30a-LI,并转化入大肠杆菌(Escherichia coli)BL21(DE3)中。
METHODS: Human arresten gene was amplified from recombinant plasmid pGEM-Arr with polymerase chain reaction (PCR), and then cloned into prokaryotic expression vector pRSET by means of recombinant gene technology. 方法 :利用聚合酶链式反应 (PCR) ,由我们构建的重组质粒pGEM -Arr中扩增出arresten基因 ;
Firstly, I subcloned the DNA fragment that encode the four consensus repeat of DAF into the prokaryotic expression vector pET32a(+) which could add a Trx tag on the N-terminal of the target protein. 首先通过PCR的方法，将编码大鼠DAF四个SCR的基因片段亚克隆到Trx融合原核表达载体pET32a(+)。
Methods The gene sequence encoding human myoglobin(hMb) from the total RNA of human skeletal muscle by RT-PCR and clone into prokaryotic expression vector pRSETc for expression in E. 方法应用RT-PCR方法从人骨骼肌总RNA中扩增肌红蛋白基因(hMb)编码序列;克隆入原核表达载体pRSETc中;并在大肠杆菌E.
Porcine parvovirus(PPV) SY_99strain was isolated by our laboratory. We extracted the genomic DNA of SY_99 strain and amplified NS1 gene. NS1 gene was cloned into pET28a,a prokaryotic expression vector,and then sequenced. 猪细小病毒 (Porcineparvovirus,PPV)SY_99株由本室分离 ,提取SY_99株的基因组DNA ,利用PCR扩增出全长NS1基因 ,将该基因克隆到原核表达载体pET2 8a中并测序。
Meanwhile, the feline IL-18 gene was subcloned into the prokaryotic expressing vector pET28a and recombinant vector was further transformed into Escherichia coli strain BL21(DE3) for expression under the induction of IPTG. 将目的基因片段进一步亚克隆到大肠杆菌(Escherichia coli)表达载体pET28a中构建了重组质粒pETIL-18,转化大肠杆菌BL21(DE3),并用IPTG诱导。
HER 2/neu receptor ligand domain 2 (RLD2) cDNA was amplified by PCR, cloned into the prokaryotic expression vector containing thioredoxin A (TrxA) gene and expressed in soluble form as TrxA RLD2 fusion protein. 用PCR技术扩增HER 2 neu胞外配体结合区 2 (RLD2 )cDNA ;并将扩增的基因片段克隆于硫氧还蛋白 (TrxA)原核表达载体中 ;获得TrxA RLD2融合蛋白的可溶性表达 .
The study aimed to construct the prokaryotic expression vectors carrying MTB esat6 and lhp and lhp-esat6 fusion genes amplified by PCR, and express them in E. coli respectively. 本研究通过体外扩增MTB esat6、lhp及lhp-esat6融合基因，构建原核表达载体并在E.
The recombinant plasmid pMD-ORF2 was digested with EcoR V and Xhol I and then the fragment was sub-cloned into the prokaryotic expressing vector pET-32a(+), getting the recombinant plasmid pET-ORF2. 将重组质粒pMD-ORF2的EcoR V和Xhol I双酶切产物亚克隆到原核表达载体pET-32a(+)，构建ORF2基因重组表达质粒pET-ORF2。
Results The prokaryotic expression vector was successfully constructed and purified from E. coli.The recombinant protein was expressed and polyclonal antibody against Norpeg was prepared. 结果成功构建原核表达载体，表达了重组蛋白并制备了小鼠抗Norpeg多克隆抗体。

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