Porcine parvovirus(PPV) SY_99strain was isolated by our laboratory. We extracted the genomic DNA of SY_99 strain and amplified NS1 gene. NS1 gene was cloned into pET28a,a prokaryotic expression vector,and then sequenced.

• 猪细小病毒 (Porcineparvovirus,PPV)SY_99株由本室分离 ,提取SY_99株的基因组DNA ,利用PCR扩增出全长NS1基因 ,将该基因克隆到原核表达载体pET2 8a中并测序。