Subsequently, the P36 gene of Mhp strain ZCF23 was subcloned into the prokaryotic expression vector pGEX 6P-1 through EcoR I and Xho I double digestion, then the recombinant expression vector pGEX 6P-1-P36 was constructed and transformed into E.

• 序列确认后，用Ec口Rl和肠口I将P36基因从克隆载体中切出，并插入到GsT融和表达载体pGEx 6p一1相应位点，构建成重组表达载体pGEX 6p一1一P36，并转化宿主菌BLZI。 阳性克隆命名为BLZI(6P一l一P36)。