Full-length or truncated cDNA was subcloned into prokaryotic expression vector pET30a and expression induced in E. coli BL21(DE3).

 
  • 全长的或C末端截短的鲨烯合酶cDNA被克隆进原核表达载体pET30a并在大肠杆菌BL21(DE3)中诱导表达。
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