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- plant RNA extraction 植物RNA提取
- A Fast Method for Total RNA Extraction From the Tissue Culture Material of Malus sp. 苹果属RNA高效快速提取新方法。
- Based on the promoter characteristic of RRD3, we designed a plant universal binary vector, pCRiRRD3, which is suit- able for performing researches on plant RNA interference. 基于RRD3的双效启动子特性,我们设计并构建了植物通用双元RNAi载体pCRiRRD3,适于进行植物的RNA干涉实验研究。
- Abstract : Objective To explore a simple and efficient method for RNA extraction from vaginal secretion of patients with vulvovaginal candidiasis and to testify its feasibility. 摘要 ： 目的 探索一种简单有效的人体阴道分泌物标本总RNA提取方法并验证其可行性。
- In this system, cloned maize mitochondrial DNA(mtDNA) is served as template. this is the first established cell-free system for in vitro transcription of cloned DNA with a plant RNA polymerase B. 这是首次用克隆化DNA为模板，以植物RNA聚合酶B建立起的无细胞离体转录系统。
- The modified CTAB and RNAplant Kit methods were a bit short in total RNA extraction because of a little DNA contamination. And the RNAplant Kit method showed advantages of fastness and convenience. 改良CTAB法和RNAplant试剂盒法提取的RNA均有少量DNA污染,但RNAplant试剂盒法比CTAB法更快捷,简便。
- We compared the quality of total RNA extracting by five methods, CTAB, TRIZOL, WI, GT, and TRIS-SDS respectively. 本试验采用了五种方法(CTAB法、TRIZOL法、WI法、GT法和TRIS-SDS法)，对三个百合种试验材料(鲜切花、卷丹、麝香百合)进行了研究。
- Main reagent : dilution fluid, hemolysis fluid, Bcr/Abl(P230) , primer and probe of GAPDH gene, primer of DCC gene, RNA extracting fluid, cDNA kit. 主要试剂：血液稀释液、溶血液、Bcr/Abl(P230)和内参GAPDH基因引物及探针、DCC基因引物、RNA提取液、cDNA合成试剂盒，乙酸钠-柠檬酸缓冲液(pH：4.;3)为流动相。
- New Methods for Total RNA Extraction in Fruit Trees 果树组织中总RNA提取的新方法
- A method of RNA extraction from grapevine bud 一种提取葡萄芽中总RNA的方法
- The plant was growing at an angle. 植物呈一定角度生长。
- The Strategy for Constructing Plant RNA Virus Vector 植物RNA病毒载体构建策略
- A Study on Methods for RNA Extraction From Citrus Tissues 柑橘组织RNA提取方法研究
- Using the total RNA extracted from ConA stimulated equine peripheral blood mononuclear cells(PBMC) as template,the cDNA of interleukin l8 was amplified by RT-PCR. The cDNA was subsequently cloned into the vector pCR2.1-TOPO and sequenced. 用RT-PCR从经ConA刺激的马外周血单个核细胞(PBMC)中扩增出马白细胞介素18(Equine interleu-kin-18;EIL-18)前体蛋白(precursor EIL-18;pEIL-18)基因的cDNA;然后克隆至载体pCR2.;1-TOPO中;鉴定并命名为pCR2
- The genes coding for hemagglutinin(H) and fusion protein(F) of measles virus were obtained from viral RNA extracted from supernatant of viral culture by RT PCR,and were cloned into baculovirus transfer vectors,respectively. 采用逆转录－多聚酶链反应（RT－PCR）的方法从麻疹病毒Edmonston株基因组中扩增出血凝素H基因和融合蛋白F基因，并利用转移质粒将这两个基因分别重组到杆状病毒多角体蛋白启动子（PH）控制之下，获得重组病毒vBMVH和vBMVF。
- Methods The cDNA was retrotranscripted from RNA extracted from OFT tissues of both Cx43 knockout and Cx43 wild type mouse embryos on embryonic day (ED) 14.5. The biotin-labeled cRNA derived from the transcription of cDNA was fragmented as probes. 方法以胎龄(embryonic day;ED)14.;5天的Cx43KO和野生型(Cx43WT)鼠胚心脏近端流出道部分为研究对象;分别提取总RNA;逆转录成cDNA; 并在体外转录为cRNA;同时进行生物素标记及片段化;
- The plant converts crude oil into gasoline. 这家工厂将原油加工成汽油。
- Methods Total RNA extracted from rat brian cell was transcripted reversely into cDNA with random primers. The Glutamic acid decarboxylase 67 gene fragments were amplified using PCR method and sequenced using the dideoxy chain termination technique. 方法 采用RT -PCR方法 ;大鼠脑组织中的mRNA逆转录成cDNA ;再以cDNA为模板 ;扩增谷氨酸脱羧酶GAD6 7基因片段 ;克隆入T载体 ;并经序列测定 .
- Now ordinary repairs can be handled in our plant. 现在一般的修理工作都可在我们厂进行了。
- Two methods of different RNA extractions from Conus venom tube and toxin gland of seven cone snail species were performed. ThermoScript~ TM RNase H~ - Transcriptase and AMV Transcriptase were used to synthesize cDNA separately. 采用不同的RNA提取方法,从7种海南产芋螺的毒管中分离总RNA,用两种反转录酶分别合成cDNA,再以合成的cDNA为模板进行PCR扩增。
