Using the total RNA extracted from ConA stimulated equine peripheral blood mononuclear cells(PBMC) as template,the cDNA of interleukin l8 was amplified by RT-PCR. The cDNA was subsequently cloned into the vector pCR2.1-TOPO and sequenced.是什么意思_Using the total RNA extracted from ConA stimulated equine peripheral blood mononuclear cells(PBMC) as template,the cDNA of interleukin l8 was amplified by RT-PCR. The cDNA was subsequently cloned into the vector pCR2.1-TOPO and sequenced.在线翻译_英语_读音_用法_例句