Methods The cDNA was retrotranscripted from RNA extracted from OFT tissues of both Cx43 knockout and Cx43 wild type mouse embryos on embryonic day (ED) 14.5. The biotin-labeled cRNA derived from the transcription of cDNA was fragmented as probes.

• 方法以胎龄(embryonic day；ED)14.；5天的Cx43KO和野生型(Cx43WT)鼠胚心脏近端流出道部分为研究对象；分别提取总RNA；逆转录成cDNA; 并在体外转录为cRNA；同时进行生物素标记及片段化;