The genes coding for hemagglutinin(H) and fusion protein(F) of measles virus were obtained from viral RNA extracted from supernatant of viral culture by RT PCR,and were cloned into baculovirus transfer vectors,respectively.

• 采用逆转录－多聚酶链反应（RT－PCR）的方法从麻疹病毒Edmonston株基因组中扩增出血凝素H基因和融合蛋白F基因，并利用转移质粒将这两个基因分别重组到杆状病毒多角体蛋白启动子（PH）控制之下，获得重组病毒vBMVH和vBMVF。