Methods Total RNA extracted from rat brian cell was transcripted reversely into cDNA with random primers. The Glutamic acid decarboxylase 67 gene fragments were amplified using PCR method and sequenced using the dideoxy chain termination technique.

• 方法 采用RT -PCR方法 ；大鼠脑组织中的mRNA逆转录成cDNA ；再以cDNA为模板 ；扩增谷氨酸脱羧酶GAD6 7基因片段 ；克隆入T载体 ；并经序列测定 .