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- Transient transfection shows that GCRG213 is located in cytoplasm and nuclei of COS-7 cells. GCRG213蛋白在胞核和胞质中均有表达。
- The transient transfection of Stat 3 cDNA into COS7 cells increased Stat3 protein level and the expression of the reporter gene. Stat3cDNA和报道基因在COS7细胞中的共转染实验表明 ,外源的Stat3蛋白具有DNA结合活性 ,并可增强报道基因的表达。
- Transient transfection of Stat3 cDNA into COS7 cells increased Stat3 protein level and also the reporter gene expression. Stat3 cDNA和报告基因在COS7细胞中的共转染实验表明，外源的Stat3蛋白具有DNA结合活性，并可增强报告基因的表达。
- The mount of vectors and polybrene, the hatching time for them were optimized through transient transfection of pG-CMV-DRE(2). 通过瞬时转染，在转染了载体pG-CMV-DRE(2)和pG-SV-DRE(2)的细胞体内观察到有报告基因绿色荧光蛋白表达。
- Recombinant retrovirus was produced by transient transfection of the Phoenix-ecotropic packaging cell line with pMX-3FLAG-Id3-EGFP vector using FuGene-6 transfection reagent. 通过脂质体转染技术将重组逆转录病毒载体(pMX-3FLAG-Id3-EGFP)转染Phoenix-ecotropic包装细胞系;
- After transient transfection into K562 cells, real-time quantitation PCR was used to detect the expression alteration of PCGF4/Bmi-1, and growth curve was utilized to observe the alteration of growth rate of cells. 转染K562细胞,瞬时干扰PCGF4/Bmi-1基因的表达,实时定量PCR检测干扰前后基因表达变化。 利用生长曲线测定干扰载体转染前后细胞生长速度变化。
- After transient transfection of COS 7 cells with mixture of equal amount of the two plasmids, the antigens JEV E and HBV preS1 were both detected in the culture supernatant by ELISA with corresponding monoclonal antibodies. 等量混合这两种质粒，转染COS?7细胞进行瞬间表达，应用单克隆抗本ELISA法检测特异性抗原。
- Method:p-AAV-CD151 was construted and was used to transiently transfect to HUVEC mediated with lipofectamine 2000. After transfection, the expression of CD151 were measured by western blot. Proliferation of HUVEC were measured by MTT colorimetric assay. 方法:通过构建p-AAV-CD151质粒并瞬时转染HUVEC,Western Blot检测CD151表达,采用MTT法测定HUVEC增殖的变化。
- Her feeling of depression was transient. 她那抑郁的心情一会儿就过去了。
- Keywords RNA editing deaminase,cellular translocation,transient transfection,Western blotting,isoforms of protein,ADAR1; RNA编辑酶;细胞内转位;瞬时转染;免疫印迹;蛋白亚型;ADAR1;
- Western blot showed that hMGRAP protein was massively secreted out from the transiently transfected Cos-7 cells. Western blot实验表明hMGRAP能大量地从瞬时转染的cos-7细胞中分泌到细胞培养液中。
- The bioactivity of IL 6 in culture supernatants of COS 7 cell transiently transfected by the new vectors was detected with IL 6 dependent cell line 7TD1 by MTT method. 将它们瞬时转染COS?7细胞后，利用IL?6依赖株7TD1对上清中的IL?6进行检测。
- Othermore, plasmid phIgVhB7DC was transiently transfected into the 293T cells, and hB7DChIg fusion protein was obtained for monoclonal antibody preparation and screening. 随后，通过将质粒phIgVhB7DC瞬时转染293T细胞，收获hB7DChIg融合蛋白用于单克隆抗体的制备和筛选。
- transient transfection 瞬时转染
- transient transfection assays 瞬时转染分析
- The recombination plasmid was transiently transfected into COS7 cells and the expression of the fusion protein was identified by RT PCR, immunofluorescent assay and sandwich ELISA. 以其瞬时转染COS7细胞 ,应用RT PCR、细胞免疫荧光技术 ,及夹心ELISA检测融合蛋白的表达。
- The GAP-43 gene was inserted into eukaryotic expression vector pEGFP-N3.The recombinant expression vector was transiently transfected into COS-7 cells by LipofectamineTM 2000 reagent. 以LipofectamineTM2000试剂转染pEGFP-N3-GAP-43表达载体至COS-7细胞中进行瞬时真核表达。
- The moods were many and transient (W.H. Hudson). 心绪纷繁但往往如过往烟云 H 赫德森）。
- For (ii) the transient response is required. 对（ii）则需要求瞬态响应。
- "The moods were many and transient" (W.H. Hudson). “心绪纷繁但往往如过往烟云” (W·H·哈得孙）。
