Method:p-AAV-CD151 was construted and was used to transiently transfect to HUVEC mediated with lipofectamine 2000. After transfection, the expression of CD151 were measured by western blot. Proliferation of HUVEC were measured by MTT colorimetric assay.

 
  • 方法:通过构建p-AAV-CD151质粒并瞬时转染HUVEC,Western Blot检测CD151表达,采用MTT法测定HUVEC增殖的变化。
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