Othermore, plasmid phIgVhB7DC was transiently transfected into the 293T cells, and hB7DChIg fusion protein was obtained for monoclonal antibody preparation and screening.

• 随后，通过将质粒phIgVhB7DC瞬时转染293T细胞，收获hB7DChIg融合蛋白用于单克隆抗体的制备和筛选。