After transient transfection of COS 7 cells with mixture of equal amount of the two plasmids, the antigens JEV E and HBV preS1 were both detected in the culture supernatant by ELISA with corresponding monoclonal antibodies.

• 等量混合这两种质粒，转染COS?7细胞进行瞬间表达，应用单克隆抗本ELISA法检测特异性抗原。