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- LCB1 gene was cloned into inducible expression vector pYES2 and transformed into Saccharomyces cerevisiae FY2 The plasmid was induced to express with galactose. 酿酒酵母 (Saccharomycescerevisiae)LCB1 (Longchainbase)基因被克隆到酵母诱导表达载体pYES2中 ,并转入到FY2中 ,用半乳糖诱导表达。
- Constructing the fused expression vector of elp gene of Echinococcusmultilocularis Sichuan isolate and its induced expression in E. Coli 四川株多房棘球绦虫elp基因融合表达载体的构建及其诱导表达
- induced expression vector 诱导表达载体
- The cDNA of BcpLH gene was cloned into a His-fusion expression vector pET-28a (+) and was induced to express in E. colt strain BL21(DE3). 在含有His标记序列的原核表达载体pET28-a(+)上插入Bc-pLH基因的cDNA,在大肠杆菌BL21(DE3)中诱导表达出了特异性蛋白,并免疫大白兔制备出高效价的抗血清;
- Full-length or truncated cDNA was subcloned into prokaryotic expression vector pET30a and expression induced in E. coli BL21(DE3). 全长的或C末端截短的鲨烯合酶cDNA被克隆进原核表达载体pET30a并在大肠杆菌BL21(DE3)中诱导表达。
- Fig.1.Construction of the shuttle expression vector pRL_hEGF. 标题： 图1.;穿梭表达载体pRL_hEGF的构建。
- ZP epitope vaccines expression vector were constructed. Recombinant proteins induced immunological responses. T he infertility percentage of ZP3,ZP2and ZP3-ZP2is60.00%,50.00%and 83.33%respectiv ely. 构建了ZP表位疫苗载体;成功地诱导出体液免疫应答;ZP3、ZP2及ZP2和ZP3多表位疫苗的避孕率分别为60.;00%25、50
- The fragment containing ubi-53 gene was inserted into pET-28a expression vector, expression of the fusion protein was induced by IPTG in E. Coli BL21 (DE3) . The fusion protein was identified by Western blot with a mouse Ab against bovine Ubiquitin. 将甜菜夜蛾的ubi-53基因克隆到原核表达载体pET-28a上，转化至大肠杆菌BL21（DE3）中，用IPTG进行诱导表达，用异源泛素单克隆抗体进行Western blot检测，证明了原核表达蛋白是目的蛋白。
- The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功构建pPIC9K/Ang-1毕氏酵母表达载体。
- Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。
- Methods:The full-length gene of NY-ESO-1 was generated by gene splicing method and the recombinant expression vector NY-ESO-1-pET-28a (+) was constructed. E. coli BL21 (DE3) bearing the plasmid was induced with IPTG for protein production. 方法:通过全基因拼接获得NY-ESO-1基因,构建重组表达载体NY-ESO-1-pET28a(+),在大肠杆菌BL21(DE3)中利用IPTG诱导获得表达,利用单克隆抗体进行Western印迹鉴定,通过Ni柱亲和纯化获得纯化蛋白。
- The mRNA of broiler HSP70 gene was amplified by RT-PCR and the production was cloned into the PGEM-T Easy vector and the expression vector pET-28a (+) . The recombinant expression vector was transformed into E. coli BL-21 and induced by IPTG to express. 利用RT-PCR方法扩增了肉鸡HSP70 mRNA,将其扩增产物克隆到PGEM-T Easy载体和原核表达载体pET-28a(+)上,进一步转化至大肠杆菌BL-21中,用IPTG诱导表达重组肉鸡HSP70。
- inducible expression vector 可调控表达载体
- After the induced expression, the protein GDH was isolated and analyzed with sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE). 它同时还是一种进化上极为保守的蛋白，是病原体种属鉴定时重要的诊断性抗原。
- The inclusion bodies were extracted by ultrasonic-breaking, and then the unique band was cut off from induced expression product separated by SDS-PAGE. 用超声波破碎细胞壁提取包涵体，SDS-PAGE结束后切下目的条带再用透析法复性浓缩，获得纯化的重组蛋白。
- The engineering strain was obtained by screening, it"s induced expression condition was optimized.The recombinant protein hIL-10 was overexpressed in E.coli stably. 筛选工程菌，优化诱导表达条件，在大肠杆菌中获得稳定高效表达。
- And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基础上再构建重组表达载体pBI-ced9，将CED-9基因置于CaMV35S启动子控制之下。
- The baculovirus expression vector pAC-HBs-Fc for complete IgG antibody against HBsAg was successfully constructed. 已成功构建表达载体pAC-HBs-Fc,为表达抗人HBsAg的IgG全抗体奠定了基础。
- The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 将该cDNA插入原核表达载体pET3d并在大肠杆菌BL21(DE3)中过量表达。
- Objective To construct the antisense expression vector of human Na + H + exchanger 1 (NHE 1). 目的 构建人Na+ H+ 交换蛋白 1(Na+ H+ exchanger 1,NHE 1)基因反义真核表达载体。
