The fragment containing ubi-53 gene was inserted into pET-28a expression vector, expression of the fusion protein was induced by IPTG in E. Coli BL21 (DE3) . The fusion protein was identified by Western blot with a mouse Ab against bovine Ubiquitin.

 
  • 将甜菜夜蛾的ubi-53基因克隆到原核表达载体pET-28a上,转化至大肠杆菌BL21(DE3)中,用IPTG进行诱导表达,用异源泛素单克隆抗体进行Western blot检测,证明了原核表达蛋白是目的蛋白。
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