The mRNA of broiler HSP70 gene was amplified by RT-PCR and the production was cloned into the PGEM-T Easy vector and the expression vector pET-28a (+) . The recombinant expression vector was transformed into E. coli BL-21 and induced by IPTG to express.

• 利用RT-PCR方法扩增了肉鸡HSP70 mRNA,将其扩增产物克隆到PGEM-T Easy载体和原核表达载体pET-28a(+)上,进一步转化至大肠杆菌BL-21中,用IPTG诱导表达重组肉鸡HSP70。