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- Preparation of high titer antiserum for mumps virus 腮腺炎病毒抗血清的制备
- highest titered antisera 最高滴度抗血清
- We used the purified recombinant protein expressed from prokaryotic cells to immunize the New Zealand whit rabbit and achieve the high titer and superiority antisera. 以前研究发现PRRSV、IBV、MHV和TGEV等冠状病毒的N蛋白都定位在细胞质与核仁中,这可能是冠状病毒的共有特征。
- Results High titer of antinuclear antibodies were found in the serum of his farther. 结果患儿父亲血清中有高滴度的抗核抗体。
- Results A hybridoma cell line secreting McAb steadily with high titer was obtained, which was named SU-2000. 结果 得到1株稳定分泌抗体、效价高的杂交瘤细胞株，命名为SU-2000。
- ELISA demonstrated that the highest titer of IgY against Vibrio parahaemololyticus in the high and low dose group could reach to 1：57600 and 1：28800 respectively. 用建立的ELISA方法跟踪检测免疫鸡蛋的效价，高、低剂量组最高效价分别为1：57600和1：28800；
- The rescued VSV was characterized by indirect immune fluorescence and electron microscope, it kept the similar fast and high titer growth with parent Indiana strain. 通过免疫荧光、电镜观察、RT-PCR及生长动力学研究,证实救获病毒保持典型的VSV印第安纳株特点。
- Conclusion: 1.The prediction of antigen epitope is accurate. High titer antibodies of anti-GPBB were obtained with the compound peptide immuning animals. 结论：1．本文以多参数预测的抗原表位比较准确，以此为根据合成的复合肽抗原免疫动物制备了高效价的抗体，抗体即可与天然抗原反应，也具有高度的特异性。
- Using highly pure C-reactive protein(CRP) to immunize rabbits, a high titer anti-CRP anti-serum was obtained. 采用高纯度的C反应蛋白 (CRP)免疫兔 ,获取高效价的CRP抗体。
- With the rising of HBsAg titer, positive ratio of HBeAg and HBV DNA (PCR) rises obviously. However, when in low titer, there still be positive of the last two index; when in very high titer, the positive ratio of the last two index only exceeds 70%. 结果 :随HBsAg滴定度升高 ,HBeAg和HBV DNA(pcr)阳性率亦明显升高 ,但低滴定度时仍有部分被检者后两项呈阳性 ,而高滴定度时后两项的阳性率仅略高于 70 %25。
- These findings indicated that the functions of EBV activated B lymphocytes of NPC patients and high titer of IgA/VCA individuals were lower than low titer of IgA/VCA and negative individuals in early immunological response of EBV infected PBMC. 结果表明;在EBV体外感染PBMC所引起的早期免疫反应中;NPC患体者和IgA/VCA高滴度者EBV活化的B细胞功能较IgA/VCA低滴度和阴性者降低.
- The analysis of MALDI-TOF-MS demonstrated that molecular weight is 39.4kD. The HPT protein was used to immunize rabbit for the preparation of polyclonal antibodies with high titer. MALDI-TOF-MS分析表明;HPT蛋白分子量为39.;4KD。
- Remarkable immunogenicity of the TrxA/PEB1 fusion protein was well identified: A high titer of the specific antibody to CJ Pen19 whole antigen weredemonstrated, which could cross-reacted with purified TrxA/PEB1 fusion protein. （3）证实TrxA/PEB1融合蛋白有高度特异的免疫原活性： CJ Pen19全菌抗原免疫后4周在雄性新西兰兔诱发高滴度特异性抗体，可同时与Pen19菌体粗提蛋白和TrxA/PEB1蛋白发生特异性结合。
- High titer serum and immunoglobulin G (IgG) were prepared from specific pathogen free (SPF) chickens immunized with inactivated AIV subtype H9N2. Rabbits were immunized with this chicken anti-H9N2-AIV IgG and high titer serum were collected. 将H9N2亚型AIV增殖,经差速离心和蔗糖密度梯度离心提纯后,灭活,免疫SPF鸡,制备高免血清并提纯免疫球蛋白G(IgG)。 以此制备疫苗免疫家兔,制备高免血清并提纯其中的IgG。
- The prepared IgY antibodies showed high titers,specificities and stabilities. 制备的抗3种咽喉菌IgY抗体滴度高,特异性强,稳定性好。
- Previously we found that DHF patients’ sera contain higher titer against TNF receptor families, including TRAIL-R1 (DR4). 先前我们的研究中发现DHF病人血清中能交互作用到肿瘤坏死因子群中的DR4。
- Inthis study ,Rabies virus cell adapted strain SRV9 , were inoculated in suckling mouse brains to expand anddetected by RT-PCR . The results of TCID50 indicated that there are high titer virus in brains. 本实验将RV SRV9 毒株接种乳鼠,发病后收集鼠脑,经匀浆离心收取上清,RT-PCR 分析,TCID50测定证明,产物中含有高效价狂犬病病毒。
- The antiserum had a titer of 1/1024 with high specificity to TRSV. 以此融合蛋白制备的抗血清的效价为1/1024，抗血清与TRSV具有良好的特异性反应。
- The SPF chickens were incubated with oil-adjuvanted inactivated AIV vaccine, then the high titer serum was collected and the Immunoglobulin G was extracted with the saturated (NH_4)_2SO_4.And the content of the IgG is 4.090mg/mL. 将该病毒抗原制成油佐剂灭活疫苗;免疫SPF鸡;收集高免血清;用(NH_4)_2SO_4法提取血清中的免疫球蛋白G(IgG);测定蛋白含量为IgG浓度为4.;090mg/mL。
- Less time was used for the rabbits to produce the PAbs with a higher titer and production, so the rabbit was chosen to produce PAbs in the subsequent experiments. 其中兔抗体制备的时间较短,效价高,且抗体的总量大,因此在十几种赤潮藻的多克隆抗体制备过程中,采用家兔作为接受免疫的对象。
