您要查找的是不是:
- Objective: To construct the recombinant expression vector of genes encoding light chain of antibody against Mumps Viruses. 目的:构建抗腮腺炎病毒抗体轻链基因重组表达载体。
- A gene fragment of about 450 bp was amplified by PCR and the recombinant expression vector pGEX4T1-CRD was constructed, whose restriction maps and sequence were consistent with those of expected one. PCR扩增得到长约450bp的目的基因片段,插入pGEX4T1载体所获重组表达载体pGEX4T1CRD的酶切图谱和序列与预期的一致。
- Subsequently, the P36 gene of Mhp strain ZCF23 was subcloned into the prokaryotic expression vector pGEX 6P-1 through EcoR I and Xho I double digestion, then the recombinant expression vector pGEX 6P-1-P36 was constructed and transformed into E. 序列确认后,用Ec口Rl和肠口I将P36基因从克隆载体中切出,并插入到GsT融和表达载体pGEx 6p一1相应位点,构建成重组表达载体pGEX 6p一1一P36,并转化宿主菌BLZI。 阳性克隆命名为BLZI(6P一l一P36)。
- Restriction endonuclease digestive identification was right for recombinant expression vector pCD11b BFP. Blue fluorescence from fusion protein could be seen in U937 cells transfected with plasmid pCD11b BFP. 经酶切鉴定 ,p CD11b- BFP构建完全正确 ,转染 U937细胞株后 ,可见 CD11b- BFP融合蛋白发出的蓝色荧光。
- The expression vector of humanized antibody light chain gene of against HCV is constructed effectively which laya solid foundation for further constructing the recombinant expression vector of humanized antibody Fab fragment against HCV. 人源性丙肝病毒抗体轻链基因表达载体是高效的,为下一步构建丙肝病毒抗体F ab段基因重组载体奠定了基础。
- The GAP-43 gene was inserted into eukaryotic expression vector pEGFP-N3.The recombinant expression vector was transiently transfected into COS-7 cells by LipofectamineTM 2000 reagent. 以LipofectamineTM2000试剂转染pEGFP-N3-GAP-43表达载体至COS-7细胞中进行瞬时真核表达。
- Methods:The full-length gene of NY-ESO-1 was generated by gene splicing method and the recombinant expression vector NY-ESO-1-pET-28a (+) was constructed. E. coli BL21 (DE3) bearing the plasmid was induced with IPTG for protein production. 方法:通过全基因拼接获得NY-ESO-1基因,构建重组表达载体NY-ESO-1-pET28a(+),在大肠杆菌BL21(DE3)中利用IPTG诱导获得表达,利用单克隆抗体进行Western印迹鉴定,通过Ni柱亲和纯化获得纯化蛋白。
- The mRNA of broiler HSP70 gene was amplified by RT-PCR and the production was cloned into the PGEM-T Easy vector and the expression vector pET-28a (+) . The recombinant expression vector was transformed into E. coli BL-21 and induced by IPTG to express. 利用RT-PCR方法扩增了肉鸡HSP70 mRNA,将其扩增产物克隆到PGEM-T Easy载体和原核表达载体pET-28a(+)上,进一步转化至大肠杆菌BL-21中,用IPTG诱导表达重组肉鸡HSP70。
- A recombinant expressing plasmid pCMV4/TPO was also constructed by cloning the rhTPO cDNA in an eukaryotic expressing vector pCMV4. When the recombinant plasmid was used to transfect the COS7 cells, temporary expression of rhTPO was detected. 将TPOcDNA亚克隆至哺乳动物细胞瞬时表达载体pCMV4,形成了重组表达质粒pCMV4/TPO。 以该重组质粒转染COS7细胞,可测到TPO在COS7细胞中的瞬时表达
- Recombinant expression vector pET-29b-TCS pET-29b-TCS重组表达载体
- Cloning of ACC Oxidase Gene of Peach and Construction of Its Plant Recombinant Expression Vectors?? 桃ACC氧化酶基因的克隆和植物表达载体的构建
- Methods Recombinant expression vectors containing CD (cytosine deaminase)and/or TK(thymidine kinase) gene under CMV promoter were constructed successfully. The vectors were transfected to GLC-82 tumor cell lines by use of lipofectamine. 方法 分别构建了以 CMV为启动子 ;含单纯疱疹病毒胸苷激酶( HSV-TK)和 /或大肠杆菌胞嘧啶脱氨酶( Ecoli.;CD)单、双自杀基因的真核表达载体。
- For the first time to recombine ALR procaryotic expression vector, and get the pET28a-hALR recombined expressing plasmid which was tested by sequence analysis. 首次构建了重组ALR原核表达载体,获得了pET28a-hALR的重组表达质粒,并测序证实。
- Cloning Fab Fragments Against Mumps Viruses and Construction of the Recombinant Expression Vector 抗腮腺炎病毒抗体Fab段基因的克隆及重组表达载体的构建
- Construction, identification and expression of recombinant expression vector of giutathione s-transferase-platelet factor 4 fusion protein 谷胱甘肽转硫酶-血小板因子4融合蛋白表达载体的构建、鉴定与表达
- CLONING OF GENES ENCODING LIGHT CHAIN OF ANTIBODY AGAINST MUMPS VIRUSES AND CONSTRUCTION OF THE RECOMBINANT EXPRESSION VECTOR 抗腮腺炎病毒抗体轻链基因的克隆及重组表达载体的构建
- Keywords bone sialoprotein;fluorescent protein;recombinant expression vector;breast cancer cell;transfection;optimization; 骨唾液蛋白;荧光蛋白;重组表达载体;乳腺癌细胞;转染;优化;
- Cloning of recombinant human BMP2 gene in eukaryotic expression vector provide basis for BMP2's expression. 克隆获得人骨形成蛋白 2基因 ,并得到此基因的真核表达载体 ,为人骨形成蛋白 2的表达打下了基础。
- The recombinant constitutive expression vector pGAP9K-gat was constructed by inserting the aim gene into pGAP9K. PCR拼接获得250bp的目的基因序列,将目的基因克隆于pGAP9K,获得组成型表达载体pGAP9K-gat。
- Fig.1.Construction of the shuttle expression vector pRL_hEGF. 标题: 图1.;穿梭表达载体pRL_hEGF的构建。