Methods Recombinant expression vectors containing CD (cytosine deaminase)and/or TK(thymidine kinase) gene under CMV promoter were constructed successfully. The vectors were transfected to GLC-82 tumor cell lines by use of lipofectamine.

• 方法 分别构建了以 CMV为启动子 ；含单纯疱疹病毒胸苷激酶（ HSV-TK）和 /或大肠杆菌胞嘧啶脱氨酶（ Ecoli.；CD）单、双自杀基因的真核表达载体。