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- Coomassie Blue Medicinal 考马斯蓝[血浆容量指示剂]
- Generally a choice is made between using Coomassie Blue or Silver Stain depending on sensitivity desired. 通常可以根据所需要的敏感度来选择是使用考马斯亮蓝染色或银染色。
- VSMCs were lysed by cell lysis buffer and the concentration of protein was measured using Coomassie blue G250 staining technique. 裂解细胞，考马斯亮蓝G一250染色法测定蛋白浓度，使用节触stern Blotting法检测
- MBFTRJ21 is mainly composed of polysaccharide, which is determined by Anthrone reaction, Coomassie blue reaction and Elson-Morgan method. 蒽酮反应、考玛斯亮蓝、Elson Morgan反应表明 ,絮凝剂MBFTRJ2 1为粘多糖类高分子絮凝剂 ;
- The gel was visualized by silver staining or colloidal coomassie blue staining and analysised with ImageMaster 2D Elite software. 透射扫描双向电泳凝胶，所得图谱以ImageMaster 2D Elite软件进行图像分析。
- VSMCs were lysed and the concentration of protein was measured using Coomassie blue G250 staining technique. PDGFR- 3 protein expressions were determined by Western Blotting. 时，裂解细胞提取蛋白，考马斯亮蓝G一250染色法测定蛋白浓度，使用Westem Bloning法检测PDGFR一p蛋白表达量。
- After silver staining or coomassie blue staining, images were captured by scanner, and then the images were edited and matched using Imagemaster2DE analysis software. 电泳后经银染或考马斯亮蓝染色，ImageMaster双向凝胶电泳软件分析，将有意义的差异蛋白质点胶内胰蛋白酶解，经过基质辅助激光解吸电离飞行时间质谱(MALDI-TOF)鉴定。
- Silver staining was fit to ascertain protein spot in 2-DE gels, coomassie blue was fit to detect high content protein, DIGE was fit to establish difference protein expression map. 在三种蛋白质的检测方法中，硝酸银染色适合确定蛋白质点的有无，考马斯亮蓝R-250染色适合目标蛋白质为高丰度且样品量大的情况，DIGE技术适合用于差异蛋白质表达谱的分析。
- MethodsAfter gelatin-PAGE and gelatin-SDS-PAGE,the gel was incubated in Tris-HCI buffer,containing trypsin or chymotypsin,and then was stained with Coomassie blue R250. 方法材料经过明胶-PAGE和明胶-SDS-PAGE后,分别用胰蛋白酶进行酶解,考马斯亮兰染色。
- VSMCs were lysed by cell lysis buffer and the concentration of protein was measured using Coomassie blue G250 staining technique. ATi protein expression was determined by Western Blotting. 裂解细胞，考马斯亮蓝G一250染色法测定蛋白浓度，使用节范stem Blotting法检测ATI蛋白表达量。
- Cx36 also bound to PDZ1 domain of ZO-1 in mouse retina and Cx43 bound to PDZ2 domain of ZO-1 in mouse brain. Fusion protein was detected in the membranes with anti-GST and coomassie blue. 与鼠视网膜组织孵育免疫印迹显示Cx36与ZO-1的PDZ1结构域结合,而对照组Cx43与ZO-1的PDZ2结构域结合,相同膜洗脱后抗GST抗体和考马斯亮蓝染色显示GST-PDZ融合蛋白表达。
- The BMSCs were cultured with the two kinds of microspheres respectively, and the cell extension rate, proliferation, alkaline phosphatase activity and Coomassie blue staining of the cells were assayed. 采用体外细胞培养技术,将两种微球分别与细胞一起培养,通过对细胞增殖率及碱性磷酸酶活性、考马斯亮蓝的检测,观察两种微球对培养细胞的影响。
- Hypothalamic total protein (TP) of rats in each group were separated by immobilized pH gradient-based two-dimensional gel electrophoresis (2-DE), and PDQuest 7.0 software was applied to analyse 2-DE images after coomassie blue staining. 用固相pH梯度双向凝胶电泳分离各组大鼠下丘脑的总蛋白质;经考马斯亮兰染色;PDQuest7.;0软件分析。
- Methods The total proteins of hypothalamuses in the rats were separated by immobilized pH gradient-based two-dimensional gel electrophoresis. With coomassie blue staining,the 2-DE image was analyzed by PDQuest 7.0 software. 方法用固相pH梯度双向凝胶电泳分离肝阳上亢证大鼠与正常大鼠下丘脑的总蛋白质;经考马斯亮兰染色;PDQuest 7.;0软件分析。
- After the protein was stained with a mixture of coomassie blue R-250 in 10% acetic acid-30% mcthanol, SDS-PAGE analysis yield two bands when the sample was reduced with 2-ME, IgY dissociated into a heavy chain and a light chain of 70KD and 40KD. 凝胶经考马斯亮蓝染色后出现两条蛋白带，它们的分子量分别为70KD和40KD，这两条蛋白带为免疫球蛋白经SDS-PAGE变性电泳后裂解的两个亚基。
- The clear water mirrored the blue sky. 清澈的水中映出蔚蓝的天空。
- He drinks this medical tea for medicinal purposes. 他为了治病而喝这种冲剂。
- Protein in the cell layer was measured by the Coomassie blue method; 采用考马斯亮蓝方法，测定MSCs内蛋白质；
- He kept a little brandy for medicinal purposes. 他留了点白兰地作药用。
- Many plants have medicinal properties. 许多植物具有医药性能。
