Silver staining was fit to ascertain protein spot in 2-DE gels, coomassie blue was fit to detect high content protein, DIGE was fit to establish difference protein expression map.

• 在三种蛋白质的检测方法中，硝酸银染色适合确定蛋白质点的有无，考马斯亮蓝R-250染色适合目标蛋白质为高丰度且样品量大的情况，DIGE技术适合用于差异蛋白质表达谱的分析。