MethodsAfter gelatin-PAGE and gelatin-SDS-PAGE,the gel was incubated in Tris-HCI buffer,containing trypsin or chymotypsin,and then was stained with Coomassie blue R250.

• 方法材料经过明胶-PAGE和明胶-SDS-PAGE后,分别用胰蛋白酶进行酶解,考马斯亮兰染色。