After the protein was stained with a mixture of coomassie blue R-250 in 10% acetic acid-30% mcthanol, SDS-PAGE analysis yield two bands when the sample was reduced with 2-ME, IgY dissociated into a heavy chain and a light chain of 70KD and 40KD.
英
美
- 凝胶经考马斯亮蓝染色后出现两条蛋白带,它们的分子量分别为70KD和40KD,这两条蛋白带为免疫球蛋白经SDS-PAGE变性电泳后裂解的两个亚基。