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- 连续延伸PCR方法a successive PCR method
- 我们用PCR方法获得了NDV HN蛋白结构域基因HNa和HNb,并通过重叠一延伸PCR对二者进行重组,获得了HNa-L-b片段。The gene fragments HNa, HNb and HNa-L-b coding for antigenic structural domains of NDV hemagglutinin-neuraminidase were amplified from the recombinant plasmid containing full length NDV HN gene by regular PCR or overlap extension PCR with specific primers.
- 重叠延伸PCRoverlap extension PCR
- 例如,瓷砖图案会投影到地面上和墙上,使观看者看到地面顺滑地连续延伸到投影房间的外面。For example, a tile pattern could be projected onto the floor and walls such that the viewer sees a continuous floor extending well outside the boundaries of the projection room.
- 循环延伸PCRrecursive PCR
- 交错延伸PCROverlap Extension PCR
- 重叠延伸PCR法overlap extension by polymerase chain reaction
- 她已连续四年获得一等奖。She has been awarded first prize four years in succession.
- 引进了一种新的训练方法。A new training method was introduced.
- 拼接重叠延伸PCRSOE-PCR
- 他连续三次上学迟到。He was late for school three times in succession.
- 他们商议扩展业务的最好方法。They conferred on the best way to expand business.
- 她连续患伤风。She had a succession of colds.
- 太多高中老师只是采用填鸭方法灌输学生。Too many high school teachers just spoon-feed their students.
- 群山向西延伸。The mountains have a western trend.
- 该队连续三次获胜。The team notched up their third victory in a row.
- 我喜欢这一种英语教授方法。I prefer this system of teaching English.
- 连续发烧会使人元气大伤。Continual fevers weaken the vitality.
- 经重叠延伸PCR扩增，将vH和vL连接后构建的ScFv基因大约75obp;The weight of ScFv about 750bp after VH linked with VL by SOE PCR.
- 你知道其他增加变化的方法吗?What other ways do you know to add variety?
