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- 重叠延伸PCR法overlap extension by polymerase chain reaction
- 方法设计引物,采用PCR法分别扩增UreB表位多肽编码基因Uepi和LTB编码基因,重叠延伸PCR法将两段基因拼接,T-A克隆后,构建融合基因表达质粒pET-22b(+)-Uepi-LTB,经酶切鉴定后转化E.Methods Amplify the genes encoding UreB epitope polypeptide and LTB by PCR respectively and link by overlap extension PCR. After T-A cloning,the linked gene Uepi-LTB was cloned into prokaryotic expression vector pET-22b(+),and the constructed recombinant plasmid pET-22b(+)-Uepi-LTB was transformed to E.
- 重叠延伸PCRoverlap extension PCR
- 重叠延伸PCR（SOEPCR）splicing by overlap extension PCR
- 拼接重叠延伸PCRSOE-PCR
- 除法division
- 经重叠延伸PCR扩增，将vH和vL连接后构建的ScFv基因大约75obp;The weight of ScFv about 750bp after VH linked with VL by SOE PCR.
- 宾夕法尼亚Pennsylvania
- 重叠overlapping
- 表示法notation
- 比色法colorimetry
- 循环延伸PCRrecursive PCR
- 重叠延伸overlap extension
- 层次分析法analytic hierarchy process (AHP)
- 测定法mensuration
- 连续延伸PCR方法a successive PCR method
- 企业法law of enterprise
- 重叠的overlapped
- 知识产权法law of knowledge property
- 重叠延伸聚合酶链式反应overlap-PCR
