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- 结论制备的可溶性抗Fas?Fab抗体具有抗Fas抗体的特异性,为进一步的研究奠定了基础。Anti Fas Fab phage antibody displaying on phage surface and soluble anti Fas Fab showed specific binding activity to Fas by ELISA. Conclusion The successful preparation of anti Fas Fab antibody paved a way for further study and application.
- 抗Fas抗体Anti-Fas antibody
- 目的：研究抗Fas抗体对人肾小球系膜细胞的致凋亡作用，探讨Fas?FasL在肾脏损伤中的作用。Objective:To investigate the mechanism inducing apptosis of human glomerular mesangial cells (Mcs) by anti Fas antibody.
- 抗Fas抗体诱导维甲酸分化后的髓母细胞瘤细胞凋亡All-trans retinoic acid sensitizes human medulloblastoma cells Med-3 to anti-Fas antibody mediated apoptosis
- 用重组人Fas抗原进行4轮筛选,得到100%的富集,说明Fas重组抗原富集了抗Fas?Fab噬菌体抗体,经ELISA检测均有抗Fas抗体的特异性。The phage displaying antibody fragments were subjected to four rounds of panning with recombinant Fas antigen at solid phase. The eluted phage was enriched for nearly 100 fold and the percent of recombinant clones increased from 27%25 to 100%25 after four rounds of panning.
- Fas抗体Anti-Fas antibody
- 抗Fas单抗anti-Fas monoclonal antibody
- 结论:EJ细胞系对Fas抗体触发细胞凋亡的敏感性依赖于细胞表面Fas的表达水平。the sensitivity of EJ cell line to Fas mediated apoptosis is mainly dependent upon the expression of Fas.
- 拮抗antagonism
- 拮抗剂antagonist
- 抗Fas锤头状核酶阻抑小鼠急性粒-单核白血病细胞免疫逃逸的研究Depressing the Immune Escape of Acute Myelomonocytic Leukemia via an Anti-Fas Ribozyme
- 经10－6mol/LATRA或9?cisRA分别预处理4天后，HL?60细胞Fas表达水平及对抗Fas抗体诱导凋亡的敏感性均显著提高。After incubation of HL-60 cells with 10~-6?mol/L ATRA or 9?cis RA for 4 days, both the Fas expression and AFIA were significantly enhanced.
- 目的 研究细胞表面Fas 表达水平与抗Fas 治疗生物学效应之间的关系。Objective To investigate the relationship between expression level of Fas and anti Fas biological responsiveness.
- 目的：探讨全反式维甲酸（ATRA）和9?顺式维甲酸（9?cisRA）对HL?60细胞Fas表达水平及维甲酸对抗Fas抗体诱导的细胞凋亡敏感性的影响。Objective: To investigate the effect of all?trans retinoic acid (ATRA) and 9?cis retinoic acid (9?cis RA) on the expression of Fas and the sensitivity to anti?Fas induced apotosis in HL-60 cells.
- 方法小儿急淋白血病细胞分别与Bcl?2反义脱氧寡核苷酸、抗Fas单抗培养，或与两者一起培养，再分别定量检测其凋亡细胞。Methods The percentage of apoptotic cells was detected after childhood ALL cells were incubated with Bcl 2 ASON, anti Fas MoAb or with both of them, respectively.
- 集鱼设备
- 在无FasL表达情况下 ,抗Fas单克隆抗体能诱导RMA细胞凋亡。Apoptosis could be observed without the expression of FasL when anti Fas antibody was added to RMA cells.
- 白癜风患者血清中抗黑素细胞膜表面抗原自身抗体的检测Detection of Auto Antibodies to Melanocyte Surface Antigen in Sera of Patients with Vitiligo
- 抗CD3抗体anti-CD3 antibody
- 抗Clq抗体anti-Clq antibody
