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- 结论制备的可溶性抗Fas?Fab抗体具有抗Fas抗体的特异性,为进一步的研究奠定了基础。Anti Fas Fab phage antibody displaying on phage surface and soluble anti Fas Fab showed specific binding activity to Fas by ELISA. Conclusion The successful preparation of anti Fas Fab antibody paved a way for further study and application.
- Fas抗体Anti-Fas antibody
- 抗Fas抗体Anti-Fas antibody
- 目的:研究抗Fas抗体对人肾小球系膜细胞的致凋亡作用,探讨Fas?FasL在肾脏损伤中的作用。Objective:To investigate the mechanism inducing apptosis of human glomerular mesangial cells (Mcs) by anti Fas antibody.
- 抗Fas抗体诱导维甲酸分化后的髓母细胞瘤细胞凋亡All-trans retinoic acid sensitizes human medulloblastoma cells Med-3 to anti-Fas antibody mediated apoptosis
- 结论:EJ细胞系对Fas抗体触发细胞凋亡的敏感性依赖于细胞表面Fas的表达水平。the sensitivity of EJ cell line to Fas mediated apoptosis is mainly dependent upon the expression of Fas.
- 用重组人Fas抗原进行4轮筛选,得到100%的富集,说明Fas重组抗原富集了抗Fas?Fab噬菌体抗体,经ELISA检测均有抗Fas抗体的特异性。The phage displaying antibody fragments were subjected to four rounds of panning with recombinant Fas antigen at solid phase. The eluted phage was enriched for nearly 100 fold and the percent of recombinant clones increased from 27%25 to 100%25 after four rounds of panning.
- Fas基因Fas gene
- Fas抗原Fas antigen
- 经10-6mol/LATRA或9?cisRA分别预处理4天后,HL?60细胞Fas表达水平及对抗Fas抗体诱导凋亡的敏感性均显著提高。After incubation of HL-60 cells with 10~-6?mol/L ATRA or 9?cis RA for 4 days, both the Fas expression and AFIA were significantly enhanced.
- Fas系统Fas system
- Fas/FasLFas/FasL
- 可溶性FasSoluble Fas
- [医] 抗体ab.
- 目的:探讨全反式维甲酸(ATRA)和9?顺式维甲酸(9?cisRA)对HL?60细胞Fas表达水平及维甲酸对抗Fas抗体诱导的细胞凋亡敏感性的影响。Objective: To investigate the effect of all?trans retinoic acid (ATRA) and 9?cis retinoic acid (9?cis RA) on the expression of Fas and the sensitivity to anti?Fas induced apotosis in HL-60 cells.
- X抗体Anti-hepatitis B virus X antigen antibody
- KLH抗体anti-KLH antibody
- IgG抗体IgG antibody
- HIV抗体HIV antibody
- HI抗体HI antibody