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Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid. 目的克隆人血管紧张素转换酶2基因(ACE2),并构建其真核表达载体。
Results The bicistronic eukaryotic expression plasmid was corrected and can co - express human BMP -2 and VEGF165 mRNA in vitro. 该重组质粒能在体外同时表达BMP2及VEGF165 mRNA。
Objective: To amplify the CD and TK gene fragment and analyze the DNA sequence,and then to construct eukaryotic expression plasmids pIRES-CD and pIRES-TK and determine their expression in ACC-2 cells. 目的:运用分子生物学技术扩增CD、TK基因,进行其DNA序列分析,并构建真核表达质粒pIRES-CD及pIRES-TK,将其共同转染ACC-2细胞,为进一步研究双自杀基因对ACC-2细胞杀伤作用奠定基础。
METHODS: Two kinds of eukaryotic expression plasmids, which can separately express full-length CEP65-GST and fluorescent fusion protein CEP65-Red, were constructed, and transfected into COS7 cells by DEAE-Dextran method. 方法:构建肿瘤及胚胎特异表达基因Cep65与GST融合表达的真核表达质粒,通过DEAE-Dextran方法转染COS7细胞,分离细胞的胞浆蛋白和核蛋白,W estern blot检测CEP65在细胞中的分布情况。
The expression plasmids pSVL6R and pCMV6R,constructed by inserting sIL 6R DNA separately into the downstream of SV40 late promotor of eukaryotic expression vector pSVL and CMV early promotor of pCMV4,were introduced into COS7 cells by Lipofectin. 通过基因序列分析确定了目的基因的核苷酸序列，并进一步构建了由SV40晚期启动子和HCMV早期启动子控制的表达质粒pSVL6R和pCMV6R。用脂质体介导的方法将表达质粒转染COS7细胞，并分别在mRNA水平（斑点杂交）和蛋白水平（ELISA和Western－blot）检测sIL－6R基因在COS7细胞中的表达。
Objective To construct an eukaryotic expression plasmid containing gene coding for the hemagglutinin-neuraminidase(HN)of newcastle disease virus(NDV)oncolytic strain Italien,and then to express the protein in eukaryotic cell. 目的构建天然溶瘤型新城疫病毒(NDV)Italien株血凝素-神经氨酸酶(HN)基因的真核表达载体并进行产物表达。
Objective To construct the pEGFP|C1|K vector,an eukaryotic expression plasmid of DNA double|strand break(DSB) repair gene hKu 70 antisense RNA and provide experimental material for the studies of hKu 70 gene function and toxicology. 目的构建人DNA双链断裂 (DSB)修复基因hKu70反义RNA真核表达载体pEGFP C1 K ,为以后的hKu70基因功能和毒理学研究提供实验材料。
The recombinant eukaryotic expression plasmid,pcDNA3.1A DCN was successfully constructed and 2 cell clones positively expressing DCN were selected. 成功构建重组真核表达质粒 pcDNA3 .;1A DCN;转染MsC并筛选出 2个阳性克隆株。
Mouse marrow stromal cells (MSCs) was transfected with this bicistronic eukaryotic expression plasmid using lipofecarmin reagent The expression of BMP -2 and VEGF165 were detected by RT - PCR. 进行酶切分析及序列测定后,用脂质体体外转染小鼠骨髓基质细胞,RT-PCR检测BMP2及VEGF165的表达。结果:核酸序列测定证实重组质粒构建正确;
Yang MX, Hu Y, Shen B, et al.Construction and identification of eukaryotic expression plasmid of resistance-related chymotrypsin gene[J].Acta Nanjing Med Univ (Nat Sci), 2003, 23(2): 95-7. [7]杨明夏;胡莺;沈波;等.;抗药性相关糜蛋白酶基因真核表达载体的构建和鉴定[J]
A recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed,and can be expressed stably in the NIH3T3 cells. 成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒,并且该基因在NIH3T3细胞中获得了稳定表达。
CONCLUSION: A recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed,and can be expressed stably in the NIH3T3 cells. 结论:成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒,并且该基因在NIH3T3细胞中获得了稳定表达。
EGFP was expressed both in X4550 and COS 7 cell and its expression level was comparable with corresponding recombinant S.typhimurium X4550 (pYA3334 EGFP) and EGFP eukaryotic expression plasmid pVAX1 EGFP. 将质粒p VAXD-EGFP转染 COS-7细胞 ,EGFP可在 COS-7细胞核和胞浆表达 ,在荧光显微镜下发出强烈荧光。
Objective To construct HBVx-GFP eukaryotic expression plasmid and establish stable transfected HepG2 cell line expressing HBVx-GFP fusion protein for exploring the biological function of HBVx and its roles in carcinogenesis of hepatocellular carcinoma. 目的构建绿色荧光蛋白(GFP)与人乙型肝炎病毒(HBV)X基因的重组表达载体,建立稳定表达HBVX蛋白(HBx)与GFP融合蛋白的HepG2细胞系,以进一步研究HBx的生物学功能及其在肝癌发生中的作用。
A recombinant expressing plasmid pCMV4/TPO was also constructed by cloning the rhTPO cDNA in an eukaryotic expressing vector pCMV4. When the recombinant plasmid was used to transfect the COS7 cells, temporary expression of rhTPO was detected. 将TPOcDNA亚克隆至哺乳动物细胞瞬时表达载体pCMV4，形成了重组表达质粒pCMV4/TPO。以该重组质粒转染COS7细胞，可测到TPO在COS7细胞中的瞬时表达
The eukaryotic expression plasmid pcD85B was constructed. The recombinantplsmid was transfected into COS-7 cells and the expressed target protein was tested byRT-PCR, ELISA and dot blotting. pcD3.;1(+)、pcD85B、转染 COS-7 细胞;用 RT-PCR、ELISA 和斑点印迹的方法鉴定目的基因的表达;
To observe the in vitro expression and subcellular localization of recombinant eukaryotic expression plasmid pEGFP-C1/ SJCHGC in COS-7 cells and analyze the antigenicity of the products expressed. 观察差异表达SJCHGC蛋白重组真核表达质粒pEGFP-C1/SJCHGC在COS-7细胞中的表达和亚细胞定位;并分析其表达产物的抗原性.
Objective To construct mouse granulocyte-macrophage colony stimulating factor (mGM-CSF) gene eukaryotic expressing plasmid pcDNA3-GM-CSF, to transfect the recombinant into erythroleukemia cell line FBL-3, and identify their biological activity. 目的构建小鼠转粒细胞巨噬细胞集落刺激因子 (mGM CSF)真核表达质粒 pcDNA3 GM CSF ,转染红白血病细胞系FBL 3,并鉴定其活性。
duck interferon-α eukaryotic expression plasmid 鸭α-干扰素真核表达质粒
pED GCSF is a high eukaryotic expression plasmid in mammalian cell. pED－GCSF是一个能在哺乳动物细胞中高效表达rhG－CSF的真核表达质粒。

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