Mouse marrow stromal cells (MSCs) was transfected with this bicistronic eukaryotic expression plasmid using lipofecarmin reagent The expression of BMP -2 and VEGF165 were detected by RT - PCR.

• 进行酶切分析及序列测定后,用脂质体体外转染小鼠骨髓基质细胞,RT-PCR检测BMP2及VEGF165的表达。 结果:核酸序列测定证实重组质粒构建正确;