To provide a more convincing positive control for our system, we used paromomycin which suppresses stop codons by reducing elongation fidelity. 为使我们的系统拥有一个更具说服力的阳性对照,我们使用了能通过降低翻译延长精确度来抑制终止密码子作用的巴龙霉素。
A protein called a release factor binds directly to the stop codon, causing the addition of a water molecule instead of an amino acid to the polypeptide chain. 一种叫做释放因子的蛋白质直接结合在终止密码子上,导致一个水分子而不是氨基酸被加在肽链末端。