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- shRNA真核表达载体shRNA eukaryotic expressing vector
- [方法]针对cyclin E基因,设计1对寡核苷酸,形成双链后将其将其插入Psilencer2.1-U6-neo质粒,构建cyclin E shRNA真核表达载体,酶切和测序鉴定。[Methods] Oligonucleotides were designed specific for cyclin E gene,after annealing the formed double-stranded DNAs were ligated with Psilencer2.1-U6-neo. The expression vectors of Psilencer2.1-U6/shRNA were identified by enzyme digestion and sequence analysis and transfected into MCF-7 cells.
- 方法构建Survivin基因shRNA真核表达载体pRNAT-U6.1/neo-survivin,转染人膀胱癌细胞T24并获得稳定的单克隆细胞。Methods Survivin-targeting shRNA eukaryotic expression vector pRNAT-U6. 1/neo-survivin was constructed and identified. Human bladder cancer cell T24 was transfect-ed with pRNAT-U6. 1/neo-survivin and then stable monoclonal cells were selected.
- 真核表达载体构建Expression vector
- 荧光真核表达载体Fluorescent eukaryotic expressing vector
- 以新霉素抗性基因突变体为筛选标志的真核表达载体的构建Construction of Eukaryofic Expression Vector Using Neomycin-resistance Gene Mutant as Selectable Marker
- HGF非融合蛋白真核表达载体的构建及其在骨骼肌细胞中的表达Construction of HGF Gene Non-fusion Expression Vector and Expression of HGF Protein in Skeletal Muscle Cells
- 真核细胞表达载体eukaryotic expression vector
- 人端粒酶逆转录酶基因启动子区的克隆及其真核表达载体的构建Cloning of human telomerase reverse transcriptase gene promoter and construction of its eukaryotic expression vector
- BCR/ABL特异性siRNA真核表达载体构建及其对K562细胞的影响Construction of Eukaryotic Expression Vector of SiRNA Specific for BCR/ABL Fusion Gene and Its Effects on K562 Cells
- 重组真核表达载体Recombinant human eukaryotic vector
- 真核表达载体eukaryotic expression vector
- 促凋亡分子Bad真核表达载体构建及其在人基底细胞癌细胞中的表达Construction of human Bad gene eukaryotic expressing vector and Bad gene expression in human basal cell carcinima cell line
- 真核表达质粒载体Eukaryotic expression plasmid
- 真核表达载体pEGFP-C3eukaryotic expression vector pEGFP-C3
- pcDNA3.0-PTEN真核表达载体pcDNA3.0-PTEN vectors
- CEA真核表达载体的瞬时表达及检测Transient expression of eukaryotic expression plasmid CEA in vitro and its detection
- APP基因真核表达载体的构建与鉴定The construction of the eukaryotic expression vector of mutation APP gene and its identification
- pEGFP-N1真核表达载体Eukaryotic expression vector pEGFP-N1
- 真核表达载体的构建及表达Construction and expression of human anti-HAV Fab eukaryotic expression vector
