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- 真核表达载体pEGFP-C3eukaryotic expression vector pEGFP-C3
- 成功构建hKu70基因反义RNA真核表达载体pEGFP C1 K ,为建立该基因低表达细胞株、DNA双链断裂修复缺陷和有关毒理学研究提供工具The efficient expression vector of hKu 70 gene antisense RNA,pEGFP|C1|K has been constructed successfully,it can be used to establish the human cell line which express lower hKu 70 protein and in researches about DSB repair deficiency and toxicology.
- 方法:采用基因克隆技术,将合成的短发卡样特异性PTI1(PC3)RNA干扰寡核苷酸序列插入真核表达载体pEGFP/U6,构建PTI1(PC3)shRNA的真核表达载体,体外转染人前列腺癌PC3细胞,48h后观察细胞生物学变化;METHODS: The shRNA specific to PTI-1 (PC-3) was synthesized and inserted into pEGFP/U6 by gene recombination technology to construct the expression vector, pEGFP/U6-mPs, which was then transfected into the cultured PC-3 cells.
- 目的 构建人DNA双链断裂 (DSB)修复基因hKu70反义RNA真核表达载体pEGFP C1 K ,为以后的hKu70基因功能和毒理学研究提供实验材料。Objective To construct the pEGFP|C1|K vector,an eukaryotic expression plasmid of DNA double|strand break(DSB) repair gene hKu 70 antisense RNA and provide experimental material for the studies of hKu 70 gene function and toxicology.
- pEGFP-N1真核表达载体Eukaryotic expression vector pEGFP-N1
- 经测序证实,GCRG213正确克隆入真核表达载体pEGFP-Nl,组成阳性重组子pEGFP-Nl-GCRG213。 测序正确的pEGFP-N1-GCRG213重组子经脂质体转染COS-7细胞,激光共聚焦显微镜观察GCRG213蛋白在胞核和胞质中均有表达。DNA sequence analysis showed that the GCRG213 sequence was right in the recombinant plasmid pEGFP-N1-GCRG213, transiently transfecting the eukaryotic cell COS-7 with pEGFP-N1-GCRG213. By using the confocal technique, GCRG213 was identified to be expressed in the cytoplasm and nuclei of COS-7 cells.
- 以新霉素抗性基因突变体为筛选标志的真核表达载体的构建Construction of Eukaryofic Expression Vector Using Neomycin-resistance Gene Mutant as Selectable Marker
- HGF非融合蛋白真核表达载体的构建及其在骨骼肌细胞中的表达Construction of HGF Gene Non-fusion Expression Vector and Expression of HGF Protein in Skeletal Muscle Cells
- 人端粒酶逆转录酶基因启动子区的克隆及其真核表达载体的构建Cloning of human telomerase reverse transcriptase gene promoter and construction of its eukaryotic expression vector
- BCR/ABL特异性siRNA真核表达载体构建及其对K562细胞的影响Construction of Eukaryotic Expression Vector of SiRNA Specific for BCR/ABL Fusion Gene and Its Effects on K562 Cells
- 真核表达载体构建Expression vector
- 真核表达载体eukaryotic expression vector
- 促凋亡分子Bad真核表达载体构建及其在人基底细胞癌细胞中的表达Construction of human Bad gene eukaryotic expressing vector and Bad gene expression in human basal cell carcinima cell line
- 荧光真核表达载体Fluorescent eukaryotic expressing vector
- 真核表达质粒载体Eukaryotic expression plasmid
- pcDNA3.0-PTEN真核表达载体pcDNA3.0-PTEN vectors
- shRNA真核表达载体shRNA eukaryotic expressing vector
- 重组真核表达载体Recombinant human eukaryotic vector
- 以LipofectamineTM2000试剂转染pEGFP-N3-GAP-43表达载体至COS-7细胞中进行瞬时真核表达。The GAP-43 gene was inserted into eukaryotic expression vector pEGFP-N3.The recombinant expression vector was transiently transfected into COS-7 cells by LipofectamineTM 2000 reagent.
- 真核表达载体的构建及表达Construction and expression of human anti-HAV Fab eukaryotic expression vector
