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- Construction and characterization of recombinant adenoviruses expressing biologically active human brain-derived neurotrophic factor and neurotrophin-3. [3]Chen Q(陈 谦); Wang JZ(汪家政); Liu H(刘 红); Wu Y(吴 燕); Fan M(范 明).
- The recombinant adenoviruses encoding CD/TK gene under the control of VEGFP could be efficiently generated by using the AdEasier-1 system and presented wonderfully in vitro anti-tumor effect. 应用AdEasier1系统可以成功制备含VEGFP驱动CD/TK自杀基因的重组腺病毒,该重组体具有明显的肿瘤杀伤作用。
- PCR showed that recombinant adenoviruses contained EWS/FLI1 cDNA after the PBMC were transfected by Ad EWS/FLI1,the EWS/FLI1 mRNA and its expression were confirmed by RT-PCR and immunohistology. 同源重组后产生的pADEWS/FLI1经PCR鉴定构建成功 ,纯化后的滴度为 4× 1 0 10 /ml。 转染PBMC后 ,RT -PCR证实有EWS/FLI1mRNA的转录 ,免疫检测法检测在PBMC中有EWS/FLI1的表达。
- Effects of recombinant adenoviruses carrying human urokinase-type plasminogen activator on experimental liver fibrosis 人尿激酶型纤溶酶原激活物重组腺病毒对大鼠肝纤维化的影响
- Efficient Generation of Recombinant Adenoviruses Expressing Antiangiogenic Fragment of Human Thrombospondin1 人凝血酶敏感蛋白1抗血管生成活性片段重组腺病毒高效制备
- Inhibitory effects of endostatin-vascular endothelial growth inhibitor recombinant adenoviruses on neovascularization 内皮抑素-血管内皮细胞抑制因子重组腺病毒对新生血管的抑制效应
- The Development and Characterization of Recombinant Adenoviruses Encoding RA538, p53 or p16 for Gene Therapy of Cancer 介导RA538、p53和p16基因转移的重组体腺病毒的构建及抑制肿瘤活性的研究
- Therapeutic effect of endostatin-vascular endothelial growth inhibitor recombinant adenoviruses on gastric carcinoma in nude mice 内皮抑素-血管内皮细胞抑制因子重组腺病毒对荷胃癌裸鼠的治疗
- Construction of recombinant adenoviruses carrying human urokinase-type plasminogen activator and its expression in hepatic stellate cells in vitro 人尿激酶型纤溶酶原激活剂重组腺病毒的构建及其在肝星形细胞中的表达
- recombinant adenoviruses 重组腺病毒
- [Object] To investigate the reversal effect of multidrug resistance of human hepatocellular cell (HCC) line of SMMC7721/ADM with antisense RNA of multidrug resistance gene (mdr1) delivered by recombinant adenoviruses. 目的 探讨重组腺病毒载体系统AdEasy介导的多药耐药基因(mdr1)反义RNA逆转阿霉素诱导的人肝癌耐药细胞株SMMC7721/ADM的多药耐药性的作用。
- Adenoviruses are the only enteric viruses that contain a DNA core. 腺病毒是唯一含有DNA核心的肠道病毒。
- Recombinant fragments;recombinant technology. 重组DNA碎片;重组DNA技术
- The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
- Recombinant Hepatitis C Virus NS5?Genotype 1? 重组丙型肝炎病毒NS5?基因型1?
- Nesiritide is a recombinant human BNP (rhBNP). 奈西立肽是重组人脑钠肽。
- Adenoviruses are the only enteric viruses that contain a DNA core . 腺病毒是唯一含有DNA核心的肠道病毒。
- The recombinant adenovirus is successfully constructed and can efficient express EWS/FLI1 in PBMC. 重组腺病毒AdEWS/FLI1构建成功 ,并能在PBMC中稳定有效地表达 ,为进一步进行尤文肉瘤的免疫治疗研究奠定了基础。
- Adenovirus mediated angiostatin gene therapy for rat glioma. 重组腺病毒载体介导血管抑素基因治疗大鼠脑胶质瘤的实验研究。
- Conclusion: The recombinant adenovirus generated is E1 and E3 deleted which can be further used in gene therapy in vivo. 结论:所获重组腺病毒为E1,E3缺陷型,可进一步用于基因治疗研究。