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- Prourokinase (144 411) Annexin V Chimeras fuse gene was inserted into Bombyx mori baculovirus transfer vector pBacPAK8 and co transfected with lineared DNA of Bm BacPAK6 virus into BmN cells. 将低分子量尿激酶与膜联蛋白 (AnnexinV)的融合基因重组到家蚕杆状病毒转移载体pBacPAK8中 ,获得重组转移载体pBacPAK UKAV ,并与线性化的病毒Bm PAK6DNA共转染家蚕细胞 ,获得重组病毒BacPAK UKAV。
- Methods: A recombinant cosmid containing CD and TK fusion gene was constructed, and then mixed with DNA TPC and co transfected to the 293 cells by calcium phosphate coprecipitation. 方法:构建含有CD、TK融合基因的重组粘粒,将其与腺病毒DNA末端肽复合物混合后以磷酸钙共沉淀法转染人胚肾293细胞株细胞,获取重组腺病毒,并进行鉴定。
- Chiyoda Mutual Life Insurance Co. 千代田寿险公司(日本)
- Methods:The plasmid pcDNA3 containing the wild type p16 gene and neo gene was used in the transfection experiment. 方法：采用pcDNA3质粒进行转染，该质粒含有野生型P16基因片段和新霉素抗性基因。
- Methods:The HUVEC over-expressing EL(named HUVEC-EL) was got through transfection HUVEC with plasmid pcDNA4-EL-myc/His. 方法:通过质粒转染法使HUVEC高表达EL(HUVE-EL),并与正常HUVEC对照,观察高表达EL对HUVEC的增殖、迁移和凋亡的影响。
- TNA may enhance transfection rate of plasmid DNA mediated with liposome, and may be beneficial to the treatment of cancer. TNA可促进脂质体介导质粒DNA转染结直肠癌细胞 ,将营养支持与基因治疗相结合 ,有望提高对肿瘤患者支持治疗效果。
- The enhanced green fluorescent protein(EGFP) plasmid was used as positive control and the transfection efficiency was detected by flow cytometry. 同时转染带有增强型绿色荧光蛋白(EGFP)的载体作为阳性对照,流式细胞术检测其绿色荧光以确定转染效率;
- CO is not readily perceived by the human senses. 一氧化碳不易被人的感觉器官所觉察。
- The plasmid pBV220-Arr. could be expressed in E. 成功构建的重组质粒pBV220-Arr在E.
- The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
- METHODS:H1299(human non-small cell lung cancer cell line)cells were co-transfected with recombinant plasmid pEF-BOS/GST-EGFR-TKD and pBabe-puro by calcium phosphate transfection. 方法:采用磷酸钙共沉淀基因转染技术,将已构建的pEF-BOS/GST-EGFR-TKD质粒DNA与pBabe-puro质粒DNA共同导入体外培养的人非小细胞肺癌细胞系H1299中。
- I was asked to get a price reduction from your co. 我奉令来向贵公司取得降价协议。
- Value analysis was born at the General Electric Co. 通用电器公司提出了价值分析的方法。
- Conclusion The transfection of the GM-CSF plasmid in vivo could stimulate the proliferation of the pre DC of bone marrow, and enhance antigen presentation of DC. 结论GM-CSF质粒体内注射可以明显刺激诱导小鼠髓源性DC前体的增殖，增强DC的抗原提呈作用。
- Their co - op was formed in1954. 他们合作社是在1954年成立的。
- Results The constructed recombinant plasmid contained the sequence of hOPG gene.After transfection with the plasmid, active OPG protein could be expressed in C2C12 cells. 结果 经酶切和测序鉴定证实本实验构建的重组表达质粒正确，该质粒在体外转染C2C12细胞后可表达功能性OPG蛋白。
- China Data Broadcast Technology Co. 中华数据广播技术公司
- Taiwan Semiconductor Manufacturing Co. 中国台湾半导体制造公司
- Telecommunications Development Co. 电信发展公司
- There's no comparison between ABC Co. and Kit Co. ABC公司和奇特公司不能相比。
