METHODS:H1299(human non-small cell lung cancer cell line)cells were co-transfected with recombinant plasmid pEF-BOS/GST-EGFR-TKD and pBabe-puro by calcium phosphate transfection.

• 方法:采用磷酸钙共沉淀基因转染技术,将已构建的pEF-BOS/GST-EGFR-TKD质粒DNA与pBabe-puro质粒DNA共同导入体外培养的人非小细胞肺癌细胞系H1299中。