Methods: A recombinant cosmid containing CD and TK fusion gene was constructed, and then mixed with DNA TPC and co transfected to the 293 cells by calcium phosphate coprecipitation.

• 方法:构建含有CD、TK融合基因的重组粘粒,将其与腺病毒DNA末端肽复合物混合后以磷酸钙共沉淀法转染人胚肾293细胞株细胞,获取重组腺病毒,并进行鉴定。