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- Blimp-1 could help 2ME2 induce differentiation on myeloma cell lines by downregulating pax-5 mRNA and upregulating xbp-1 mRNA. Blimp-1可通过抑制pax-5 mRNA表达水平,从而提升xbp-1mRNA表达水平,促进骨髓瘤细胞分化。
- The results showed that compared with myeloma cell, marked changes were not observed in hybridoma cell in cell energy and cell appearance. 结果表明，与凯骨髓瘤细胞相比，杂交瘤细胞的细胞活力和细胞形态无明显变化；
- The chromosome number of post-fused hybridoma cell was near to the cumulative chromosome number of splenic cell and myeloma cell. 当脾细胞与凯骨髓瘤细胞融合形成杂交瘤细胞后，其染色体数目接近两亲本细胞染色体数目之和。
- We determined the serum ANA autoantibody of 253 patients, using the BAB/C mouse myeloma cell NS-1 as antigen and applying the technique of indirect fluoro-immunoassay. 本文应用小鼠（BAB/C）骨髓瘤细胞（NS－1）细胞为抗原，采用间接免疫荧光技术检测了253例血清标本中的抗核抗体（ANA），并与鼠肝细胞为载体法进行了比较。
- The average numbers of chromosomes of the two hybridoma cell lines were 98 and 101 which were consistent with the total chromosome numbers of spleen cell and myeloma cell. 染色体计数分析结果显示，两株杂交瘤细胞的平均染色体数分别为98和101，在脾细胞与 SP2/0 细胞染色体数之和的范围之内；
- Objective:To illustrate the effect on myeloma cells with TK gene by GCV. 目的:探讨更昔洛韦对转染TK基因的骨髓瘤细胞生长的抑制作用。
- The spleen cells from the BALB/C mouse immunized with the type B toxoid were fused with aNS-1 myeloma cell line. 以B型肉毒类毒素免疫BALB/C小鼠,取脾细胞与NS-1骨髓瘤细胞进行细胞融合。
- The cTnI was used to immunize Balb/c mice by route method. The spenocytes were fused with SP2/0 myeloma cell lines. Six hybridoma cell lines,secreting stably McAbs to cTnI antigen have been obtained and screened. 方法 :应用cTnI免疫Balb/c小鼠 ,取脾细胞与SP2 /0骨髓瘤细胞进行细胞融合筛选 ,获得分泌高效价抗cTnI抗体细胞株 6株。
- SRBC-B_3 monoclone hybridema cell line is a clone derived from the fusion ofmouse myeloma cell SP 2/0 with lymphocyte B cell from spleen of BAL B/C mousewhich was immunolized with grouping A red cell. SRBC-B_3单克隆杂交细胞株是用小鼠骨髓瘤细胞 SP2/0与经过 A 型红细胞免疫BALB/C 小鼠脾脏中的 B 淋巴细胞融合而成的细胞株。
- METHODS BALB/c mice were immunized with HAV NJ 3 strain from cell culture, then lymphocytes from the splenic tissues of the mice were fused with Sp2/0 myeloma cell screening with IFA, limiting dilution technique and expanded into larger cell populations. 方法 :用已培养的 NJ-3株甲肝病毒 (HAV)免疫 BALB/c小鼠 ,经细胞融合技术、IFA筛选和有限稀释克隆法。
- After immunization of BALB/c mice with purified rabies virus(RV),three hybridoma cell strain against RV named C7,C4 and H3,respectively,were developed after fusion between SP2/0 myeloma cell and the stimulated splenocytes. 将狂犬病病毒ERA株纯化后免疫雌性BALB/c小鼠,取其脾细胞与SP2/0骨髓瘤细胞进行融合,经克隆和间接ELISA筛选,获得3株稳定分泌抗狂犬病病毒ERA株单克隆抗体的杂交瘤细胞株,分别命名为C7、C4和H3。
- They next took the spleen cells of the mice and fused them with a myeloma cell line, which yields cells that can be grown for long periods of time in the laboratory. 接下来，他们将小鼠的脾脏细胞与一种骨髓瘤细胞系融合，这样就可以产生能在实验室条件下长期存活的细胞。
- Methods:By using RV HSV TK gene system and MTT technique, the growth inhibited effect on myeloma cells with TK gene by GCV were analysed. 方法:应用缺陷型反转录病毒介导的TK基因系统(RV-HSV-TK)转染骨髓瘤细胞,采用细胞增生实验(MTT法),研究更昔洛韦对转染TK基因的骨髓瘤细胞株的抑制作用。
- The killing rates of autologous T lymphocytes induced by DC pulsed with or without myeloma antigen on myeloma cells were measured by MTT method. 结果骨髓瘤冻融物致敏DC诱导的自身T细胞对患者骨髓瘤细胞的杀伤率远大于非致敏DC。
- Hybridoma and Myeloma Cell Culture SFM 杂交瘤和骨髓瘤细胞培养专用无血清培养基
- In the present study,a hybrldoma cell line(Ell)was established by fusion of mouse myeloma cells(Sp2/0) with spleen cells from BALB/c mice immunized with HBV antigen purified from human plasma and containing a plenty of HBV Dane particles. 以富含Dane颗粒的HBV抗原作免疫原进行研究，选育出一株分泌具有抗HBc及抗HBs双重反应性McAb的杂交瘤细胞株。
- Hybridomas are prepared by the fusion of mouse myeloma cells,cell line P_3-X63-Ag8.653,and spleen cells from BALB/C mice immunized with partially clarified and concentration sap from Citrus tristeza virus(CTV) infected citron. 以感染枸橼的柑桔速衰病毒(Citrus tristeza virus;CTV)的部分纯化制备物和浓缩的感染组织粗浸出液免疫BALB/C小白鼠后获得的脾脏细胞;与鼠骨髓瘤细胞株P3-X63-Ag8.;653融合制备了杂交瘤细胞
- As a proteasome inhibitor, bortezomib has shown inhibition on cell proliferation, inducing cell apoptosis, inhibition of cell adhension and inhibition of tumor angiogenesis for multiple myeloma cells. 硼替佐米作为一种蛋白酶抑制剂，对多发性骨髓瘤细胞表现出抑制细胞生长、诱导肿瘤细胞凋亡、抑制细胞粘附、抑制肿瘤血管生成等作用，同时对血液系统其他恶性肿瘤具有显著的作用。
- A hybridoma secreting monoclonal antibody (McAb) against human antidiu-retic hormone (ADH) was obtained by fusion of Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with ADH. 应用B淋巴细胞杂交瘤技术,成功地建立了分泌抗抗利尿激素(ADH)的单克隆抗体细胞株。
- Objective:To illustrate the effect on myeloma cells with TK gene by GCV.Methods:By using RV HSV TK gene system and MTT technique, the growth inhibited effect on myeloma cells with TK gene by GCV were analysed. 目的 :探讨更昔洛韦对转染 TK基因的骨髓瘤细胞生长的抑制作用。 方法 :应用缺陷型反转录病毒介导的 TK基因系统 (RV- HSV- TK)转染骨髓瘤细胞 ,采用细胞增生实验 (MTT法 ) ,研究更昔洛韦对转染 TK基因的骨髓瘤细胞株的抑制作用。
