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- AIM: To investigate whether wheat germ agglutinin (WGA) could induce apoptosis in mouse fibroblast cell line L929 and the possible molecular mechanism underlying. 目的 :研究麦胚凝集素 (WGA)是否能够诱导小鼠成纤维细胞L92 9发生凋亡及其可能的分子机制。
- mouse fibroblast cells 小鼠纤维母细胞
- Methods:Mouse fibroblast NIH3T3 cells were transfected with 3 2 kb HBV DND by exposure of the cells to calcium phosphate. 方法:应用磷酸钙转染技术构建表达HBsAg和HBeAg的小鼠NIH3T3细胞克隆,以该细胞为模型,应用原位杂交技术测定细胞内特异性TIMPRNA含量变化。
- CONCLUSION: Mouse blastula can develop into embryonic stem cells on the fibroblast cell feeder layer, and can be passaged. 结论：小鼠囊胚在胚胎成纤维细胞饲养层上可发育成胚胎干细胞，并能进行传代。
- Keywords polylactic acid-chitin plates;L929 mouse fibroblastic cells;cytotoxicity;cell relative growth rate assay;cell morphology test; 关键词聚乳酸-甲壳素接骨板;L929小鼠成纤维细胞;细胞毒性;细胞增殖度试验;细胞形态学试验;
- The inhibition of erythroid differentiation factor (EDF), which was extracted and purified from rabbit reticulocytes, on the growth of the mouse established fibroblast cell line L929 and human nasopharyngeal cell line KB was analysed in vitro. 从兔网织红细胞提纯的红细胞分化调节因子(erythroid differentiation factor,EDF),能对体外培养的自发转化成纤维细胞系L929及人鼻咽癌细胞系KB产生作用。
- The TIMP-1 expression was only detected in the synovial lining cells and a small quantity of fibroblast cells. TIMP-1只在滑膜衬里层细胞和少量的成纤维细胞有表达,而MMP-1免疫组化显示阳性细胞数和着色强度强于TIMP-1。
- Fibroblast Cell of Adult Mouse Culture in Vitro 成年小鼠成纤维细胞体外培养
- Oxidize collagen fiber and cause lost of skin elasticity and wrinkles Cause aging of fibroblast cells thus delaying collagen production. 老化纤维细胞,危害制造胶原的机能使皮肤缺乏弹性。
- Objective The methods and outcomes of human fibroblast cell seeding on acellular porcine aorta valves were evaluated. 目的探索在脱细胞猪主动脉瓣膜材料上种植人成纤维细胞的方法与效果;
- The experiment of cryopreservation and cold storage of fibroblast cells cultured in vitro of Boer goat and yellow cattle's ear skin was conducted. 以体外培养的布尔山羊和黄牛耳组织成纤维细胞为研究对象 ,进行了哺乳动物体细胞的冻存与冷藏试验。
- Results: The cultured cells showed typical shape and features of fibroblast cell,which were successfully dissociated and purified. 结果:原代培养的成纤维细胞特征典型,并通过传代培养得到了纯化的成纤维细胞。
- Objective To observe the function of Rhodiola sacha linensis A Bor against the senility of human embryo lung diploid fibroblast cells. 目的观察红景天甙对人胚肺二倍体成纤维细胞的抗老化作用。
- Results Fibroblast cells and control group cells grew well,whereas the growth of tumour cells in experimental groups reduced gradu... 48小时癌细胞组的上清液病毒效价较高而成纤维细胞的上清液病毒效价为0。
- Objective： To study the influence of electret field on fibroblast cell cycle and to probe its mechanism. 目的:研究驻极体对成纤维细胞生长周期的影响及其调控机制。
- Cardiac extracellular matrix is made up mainly of collagen protein which is synthesized and secreted by fibroblast cells. 心脏的细胞外间质主要由成纤维细胞合成、分泌的胶原蛋白组成。
- Methods Plasmids including pDOR-neo vector,pDORHRAD17s(sense) and pDOR-HRAD17a(anti-sense) were transfected into mouse fibroblasts(NIH3T3) and human uterine cervix cancer cells(HeLa). 方法利用基因转染技术,对小鼠成纤维细胞NIH3T3和人宫颈癌HeLa细胞均分别转染pDOR-neo载体,pDOR-HRAD17s(正义)和pDOR-HRAD17a(反义)质粒,G418筛选获得阳性克隆。
- Removal of YY1 binding sites in HPV 16 LCR increases viral transforming activities on mouse fibroblasts DONG Xiaoping, LIU Hong, H. 单位：100052北京中国预防医学科学院病毒学研究所(董小平)；首都医科大学附属北京市口腔医院(刘红)；
- The paper describes the process of establishment and cryopreservation of a Giant Panda(Ailuropoda melanoleuca) skin fibroblast cell line. 简要介绍了大熊猫(Ailuropoda melanoleuca)皮肤成纤维细胞的建系过程和冷冻保存。
- Mechanisms of Fibroblast Cell Therapy for Dystrophic Epidermolysis Bullosa: High Stability of Collagen VII Favors Long-term Skin Integrity. 成纤维细胞治疗营养不良性大疱性表皮松解症机制：VII-胶原蛋白的高度稳定性有利于保持长期皮肤完整。
