Methods:Mouse fibroblast NIH3T3 cells were transfected with 3 2 kb HBV DND by exposure of the cells to calcium phosphate.

• 方法:应用磷酸钙转染技术构建表达HBsAg和HBeAg的小鼠NIH3T3细胞克隆,以该细胞为模型,应用原位杂交技术测定细胞内特异性TIMPRNA含量变化。