Methods Plasmids including pDOR-neo vector,pDORHRAD17s(sense) and pDOR-HRAD17a(anti-sense) were transfected into mouse fibroblasts(NIH3T3) and human uterine cervix cancer cells(HeLa).

• 方法利用基因转染技术,对小鼠成纤维细胞NIH3T3和人宫颈癌HeLa细胞均分别转染pDOR-neo载体,pDOR-HRAD17s(正义)和pDOR-HRAD17a(反义)质粒,G418筛选获得阳性克隆。