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- Objective To investigate the preparation methods of feeder cells of mouse embryonic stem cell. 摘要目的：探讨小鼠胚胎干细胞饲养层细胞的制备。
- The results manifested that both BRL-CM and common medium were suitable for porcine EG cells culturing and passaging when having STO feeder cells. 结果表明,以STO细胞作饲养层,使用BRL条件培养基与常规培养基在猪EG细胞分离培养、传代后均可获得细胞集落,而且使用BRL条件培养基培养产生的集落数多,细胞生长速度快。
- One EG cell line maintained undifferentiation for 6 passages. When bovine primordial germ cells were cultured on different feeder cells: MEF, STO and BEF, all can sustain bovine primary embryonic germ cells, and MEF was the best. 用不同的饲养层培养牛原始生殖细胞，实验结果表明MEF，STO和BEF饲养层都能支持牛原代PGCs的增殖，但MEF较BEF和STO效果更好以MEF、GEF、BEF为饲养层，在不添加细胞因子情况下，都可以培养出原代山羊的胚胎生殖细胞。
- In order to optimize the culture conditions, human feeder cells and feeder-free, unconditioned culture systems have been developed. 为了优化培养条件，目前人们已发展了多种人类细胞饲养层和无饲养层、非条件培养基体系。
- Feeder cells with murine embryonic fibroblasts(MEF) were better than that with human embryonic fibroblasts(hEF), or STO or bovine embryonic fibroblasts (bEF). MEF、STO、bEF和hEF可提高人原始生殖细胞(PGCs)的贴壁率,能促进PGCs源的人类胚胎干细胞(ES)的分离效率; 以MEF作为饲养层效果较好。
- When goat primordial germ cells were cultured on different feeder cells,MEF,GEF and BEF all could sustain goat primary embryonic germ cells,and MEF was finer than others. 以小鼠胎儿成纤维细胞(MEF)、山羊胎儿成纤维细胞(GEF)、牛胎儿成纤维细胞(BEF)为饲养层,在不添加细胞因子情况下,都可以培养出原代山羊的胚胎生殖细胞。
- Limbal stem cells with feeder cells grew as clones. The mean CFE of limbal stem cells in the group with feeder cells was more than that in the group without feeder cells (t=2.982,P<0.01). 与饲细胞共同培养的兔角膜缘干细胞形成细胞克隆团;细胞的平均CFE比较:有饲细胞组角膜缘干细胞明显高于无饲细胞组(t=2.;982;P<0
- When goat primordial germ cells were cultured on different feeder cells, MEF, GEF and BEF all can sustain goat primary embryonic germ cells, and MEF was finer than others, but there were no significant difference between MEF and GEF (P>0.05) . 传代结果表明，MEF的培养效果较好，但与GEF组二者差异不显著(P>0.;05)，BEF组培养效果较差（P<0
- The little boy is a noisy feeder. 这个小男孩吃东西时发出响声。
- A storage cell in some amorphous memory devices. 某些非晶体存储器中的一个存储单元。
- She was found prostrate on the floor of the cell. 有人发现她趴在小屋的地板上。
- Rescue of Dying Adherent Cells by Feeder Cells 饲养细胞用于挽救濒死的珍稀贴壁细胞
- Keywords Co irradiation;PBMC;feeder cells; 关键词60钴照射;外周血单个核细胞;饲养细胞;
- The prisoner rapped out a message on the cell wall. 那囚犯敲打囚室的墙来传达讯息。
- Keywords Feeder cells;Adherent cells;Rescue; 饲养细胞;贴壁细胞;挽救;
- The prisoner raped out a message on the cell wall. 那囚犯敲打囚室的墙来传达讯息。
- The growth of successive layers of a cell wall. 敷着，附着细胞壁连续层的生长
- The prisoner was locked in a cell. 那囚犯被关在单人牢房内。
- The prisoner paced the floor of his cell. 那犯人在牢房里来回地走著。
- The stage was designed to imitate a prison cell. 舞台设计成牢房的样子。
