One EG cell line maintained undifferentiation for 6 passages. When bovine primordial germ cells were cultured on different feeder cells: MEF, STO and BEF, all can sustain bovine primary embryonic germ cells, and MEF was the best.

• 用不同的饲养层培养牛原始生殖细胞，实验结果表明MEF，STO和BEF饲养层都能支持牛原代PGCs的增殖，但MEF较BEF和STO效果更好以MEF、GEF、BEF为饲养层，在不添加细胞因子情况下，都可以培养出原代山羊的胚胎生殖细胞。